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Phenol/chloroform Extraction of DNA
Materials:phenol:chloroform (1:1) chloroformAdd an equal volume of buffer-satura
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煮沸裂解法小量制备质粒DNA
经Triton X-100、溶菌酶和加热处理可从小量（1～2ml）细菌培养物中分离质粒DNA所获得的质粒则可以用电泳或限制性内功核酸酶消化的方法鉴定；经PEG处
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RNase Protection Assay (Bowtell Lab)
1. Cut 10ug of plasmid DNA where you want the transcript to end.2. Add an equal
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DNA Preparation from Adherent Cells
Section of Cancer Genomics,Genetics Branch,NCINational Institutes of HealthReage
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CAT ASSAY[TLC METHOD]
CAT ASSAY[TLC METHOD]1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flask
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冷冻组织DNA的提取
Tissue collection, storage, microdissection, sectioning: See separate protocol.
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DNA Extraction
This is a modification of a salting out procedure as described by Miller et al.,
2024-10-16 46 -
RAPD
一、 材料 不同来源的DNA(50ng/ul)。二、设备 PCR仪，PCR管或硅化的0.5ml eppendorf管，电泳装置。三、试剂 1、随机引
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RT-PCR对siRNA沉默效果的评价
虽然通过siRNA处理，整个基因的mRNA都受到影响，围绕siRNA周围的区域是受沉默影响最大的区域。就时效性研究来看，编码区是siRNA效应的最稳定区，非翻译
2024-10-16 52 -
CTAB TECHNIQUE / Method / Sched
CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTR
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RNase真的很顽固吗？
刚涉及RNA抽提的新手都会听到别人说RNase很顽固，用高压灭菌都无法去除其活性。于是在配制tris缓冲液时就遇到了两难的境地。tris缓冲液不能用DEPC处理
2024-10-16 46 -
Geno typing using Affymetrix arrays
Sample DNAs should not be highly degraded nor contain PCR inhibitors, such as hi
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MinElute DNA纯化技术
作分子生物学实验，核酸纯化、酶切、克隆算是最基本的步骤了。因为要做定向克隆，通常要作双酶切。为了省时省事儿，我们常常想方设法把两个酶放在一起切。可是事情并非总那
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Lambda（噬菌体）DNA Miniprep
David HarryInstitute of Forest GeneticsUSDA Forest ServicePacific Southwest Rese
2024-10-16 45 -
胚体细胞培养落叶松线粒体的DNA提取
Mitochondrial DNA Isolation from Somatic Embryogenic Cell Cultures of Larix (Exc
2024-10-16 44
