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用EBV转染淋巴细胞建立永生化细胞株的方法
一、 试剂1、 淋巴细胞分离液:避光4度保存,用前在37度水浴中加温。整个分离过程中,温度应控制在8-28度,温度过高或过低均影响分离质量。2、 全培养基(用前
2025-03-20 20 -
细胞房工作守则及注意事项
一、 常规操作1. 穿着专用实验服。自己的白大衣或厚外套,可脱在门外的衣帽架上。 2. 穿着专用拖鞋。在缓冲间换下自己的鞋子,尽量避免在缓冲间走动、停留。
2025-03-20 11 -
Telomerase Assay in Renal Cancer
Telomeres are repeating sequences located at each end of eukaryotic chromosomes.
2025-03-20 15 -
Gold Nanorods: Multifunctional Agents for Cancer Imaging and Therapy
Gold nanorods (GNRs) are strongly absorbing at near-infrared (NIR) frequencies a
2025-03-20 11 -
无白血病抑制因子条件下利用SC1(pluripotin)维持小鼠胚胎干细胞的自我更新
小鼠胚胎干细胞常规培养时需要用到白血病抑制因子(LIF),但是LIF非常昂贵。本视频将展示一种很实惠的替代方法,即以小分子SC1代替LIF维持小鼠胚胎干细胞自我
2025-03-20 12 -
Immunohistochemical Localization of TNFα and Its Receptors in the Rodent Central Nervous System
The protocols for immunohistochemical localization of tumor necrosis factor α (T
2025-03-20 13 -
Epigenetics in Myeloid Malignancies
Myeloid hematological malignancies are among the epigenetically best characteriz
2025-03-20 14 -
Detection of ras Gene Mutations Using Oligonucleotide Ligation Technology
The human ras genes (H-, K-, and N-rus ) are members of a superfamily of low-mol
2025-03-20 15 -
p53-Induced Gene Expression Analysis
Researchers in the p53 field have successfully used many high-throughput screeni
2025-03-20 16 -
N-Glycosylation Analysis by HPAEC-PAD and Mass Spectrometry
Changes in protein glycosylation are a hallmark of most types of cancer includin
2025-03-20 12 -
细胞核内显微注射DNA至游离成年哺乳动物神经细胞
详细描述了在细胞核内显微注射法注射cDNA至游离成年哺乳动物交感神经神经元的过程。 0:00 细胞核内显微注射DNA至游离成年哺乳动物神经细胞0 0:55 引言
2025-03-20 12 -
原位杂交的难题
问:各位好!我现在在大鼠脑组织的mRNA的原位杂交,测BDNF,DAB显色的时候有淡黄色,但是复染之后就是满视野蓝核了,只有零星的阳性结果,并且据文献报道以神经
2025-03-20 15 -
电穿孔
【求助】哪位大哥给指点一下电穿孔阿,xiexie参与者:vensentgwx哪位大哥给指点一下电穿孔阿,xiexie 告诉我哪里有介绍也行,我自己在网上没找到。
2025-03-20 17 -
Eradication of c-erbB-2-Positive Ovarian Cancer Cells Mediated by Intracellular Expression of Anti-c
Overexpression of erbB- 2 is important in the pathogenesis of a variety of human
2025-03-20 18 -
昆虫杆状病毒表达系统的研究进展
相关专题体外基因表达体外基因表达系统包括原核细胞系统和真核细胞系统。原核细胞系统主要是大肠杆菌细胞,它操作简便、周期短收益大及表达产物稳定,但是表达基因的相对分
2025-03-20 12