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正常人胚肺成纤维细胞的培养
实验材料:1. 肺组织:取自妊娠14—18周的胎儿;2. 不含Ca2+ 和Mg2+ 的1PBS,添加200000IU/L青霉素、200mg/L链霉素,pH7.2
2025-03-20 20 -
Production of Antibody Fragments in a Bioreactor
Clinical use of antibodies or antibody fragments is dependent on highly effectiv
2025-03-20 11 -
HSV-tk/IL-2 Gene Therapy for Glioblastoma Multiforme
We performed a clinical trial of gene therapy in patients with recurrent gliobla
2025-03-20 15 -
Electron Microscopic Assessment of Adenovirus-Mediated Transfer
This chapter describes the method of preparing and observing hepatocellular carc
2025-03-20 11 -
Epigenetics of Colon Cancer
Accumulation of genetic and epigenetic alterations transforms normal colonic epi
2025-03-20 8 -
Cationic Liposome Gene Transfer
Development of safe and effective technology for delivering functional DNA into
2025-03-20 9 -
Sphere-Forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells
Sphere-forming assays are an in vitro technique to assay both normal and neoplas
2025-03-20 11 -
跨越世纪的生命赞歌(第三部分)
863计划生物技术领域15周年回眸。影片回顾了863领域生物技术的起步、开展与成果,成果主要介绍了两系法杂交水稻、转基因抗虫棉、抗病小麦、人类基因组计划、生物技
2025-03-20 9 -
Cell Migration and the Boyden Chamber
Tumors usually reach secondary sites via blood vessels or lymphatic vessels. Two
2025-03-20 9 -
人肝癌细胞与抑癌基因的关系
Tumour suppressor p53 and Rb genes in human hepatocellular carcinoma.Aberrations
2025-03-20 9 -
转染细胞全死了的原因
问:构建逆转录病毒为载体的DNA(质粒),阳离子脂质体2000(过期的),转染病毒包装细胞pt67,G418筛选,在换液和传代过程中均会有细胞死亡(贴壁细胞漂浮
2025-03-20 10 -
Saporin Suicide Gene Therapy
New genes useful in suicide gene therapy are those encoding toxins such as plant
2025-03-20 13 -
Fluorescence In Situ Hybridization on Formalin-Fixed, Paraffin-Embedded Tissue Sections
Although in situ hybridization has been in use for over 30 years, its applicatio
2025-03-20 14 -
Preclinical Testing of Antileukemic Drugs Using an In Vivo Model of Systemic Disease
Acute lymphoblastic leukemia (ALL) is predominantly a disease of the bone marrow
2025-03-20 8 -
Detection of Mitochondrial Localization of p53
p53 is a master regulator of cell death pathways and has transcription-dependent
2025-03-20 8