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PNAS：真核生物囊泡转运机理新思路
近日来自中国科技大学和德国德国比勒费尔德大学的科研人员展开合作在新研究中首次发现酵母SNARE蛋白Vti1采用了与哺乳动物完全不同的结合位点与接头蛋白Ent3相
2025-03-20 14 -
真核细胞表达的转录水平调控
近几年来，随着分子生物学、遗传学及其新技术的迅速发展，真核生物基因表达(geexprc~ioB)的问题已有了突破性的进展。认为真核细胞基因的表达，受到多层次的调
2025-03-20 18 -
甲醇酵母系统表达的影响因素
酵母菌是单细胞真核生物，具有生长快、易于遗传操作、能对外源蛋白进行翻译后加工和修饰、不产生有毒产物等特点，被认为是表达外源蛋白的合适宿主。几种工业酵母尤其是巴氏
2025-03-20 10 -
骨髓瘤细胞的体内接种问题
【求助】骨髓瘤细胞的体内接种问题参与者：qj_82请教各位：骨髓瘤细胞如果要进行乳鼠或者大鼠的体内接种，是采用腹腔注射，还是皮下注射？还是直接注射在脾脏上？细胞
2025-03-20 9 -
[D-Arg6, D-Trp7,9, NmePhe8]-Substance P (6-11) Activates JNK and Induces Apoptosis in Small Cell Lun
[D-Arg6 , D-Trp7,9 , Nme Phe8 ]-substance P (6-11) (antagonist G) is a novel cla
2025-03-20 15 -
Generation and Screening of a Modular Human scFv Expression Library from Multiple Donors
Human monoclonal antibodies (MAbs) are more suitable than MAbs of animal origin
2025-03-20 17 -
基于细胞的酶联免疫检测实验
基于细胞的酶联免疫检测实验（Cell Based Elisa）可以：（1）检测高通量的样本，定量细胞膜上或细胞内的蛋白质的表达情况。（2）用于磷酸化蛋白质的检测
2025-03-20 9 -
Purification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins
The ability to isolate relatively pure populations of primary normal and maligna
2025-03-20 14 -
Predictive Mathematical Modeling in Metastasis
Mathematical modeling is emerging as a powerful predictive tool in many areas of
2025-03-20 10 -
Determination of Peak Serum Levels and Immune Response to the Humanized Anti-Ganglioside Antibody-In
Tumor reactive monoclonal antibodies (mAb) have been developed and tested as ant
2025-03-20 9 -
miRNA Profiling on High-Throughput OpenArray System
Micro RNA (miRNAs) are a class of 17–25 nucleotides noncoding RNAs that have bee
2025-03-20 12 -
Identification and Assembly of V Genes as Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma
Tumor-specific markers are important in identifying and tracking malignant cells
2025-03-20 12 -
A Nested RT-PCR Assay to Detect BCR/abl
Chronic myelogenous leukemia (CML), a clonal myeloproliferative disorder in adul
2025-03-20 13 -
细胞系鉴定 你 get 了么？
刚刚在中国科学院昆明细胞库看到他们今年最新公布的错误鉴定和交叉污染的细胞，共 60 个细胞系，STR 检测结果和备检细胞系的名称相差甚远，大家赶紧去看看自己的细
2025-03-20 22 -
肝素，硫酸类肝素，低分子肝素的区别
肝素（heparin）属于糖胺聚糖（glycosaminoglycans，GAGs）类生化药物，是一类在结构上不均一、聚合程度上高度分散的硫酸多糖，一般指肝素钠
2025-03-20 20
