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平板细胞克隆形成试验
概念：细胞克隆形成率即细胞接种存活率，表示接种细胞后贴壁的细胞成活并形成克隆的数量。贴壁后的细胞不一定每个都能增殖和形成克隆，而形成克隆的细胞必为贴壁和有增殖活
2025-03-20 17 -
Analyzing the Regulation and Function of ATM
We describe here the cloning of full-length ataxia-telangiectasia mutated (ATM)
2025-03-20 15 -
Chromogenic In Situ Hybridization in Tumor Pathology
Ever since the correlation was found between the pathogenesis of diseases and ge
2025-03-20 12 -
CHO细胞表达系统在疫苗研制中的应用
分子生物学、分子免疫学等学科的发展使基因工程疫苗具有越来越重要的地位。在基因工程疫苗研究的动物细胞表达系统中，最具代表性的就是中国仓鼠卵巢细胞(Chinese
2025-03-20 24 -
Southern Blotting of Genomic DNA from Lung and Its Tumors: Application to Analysis of Allele Loss on
Lung cancer is one of the most common cancers afflicting the citizens of develop
2025-03-20 17 -
RT-PCR Quantitation of HSP60 mRNA Expression
Heat shock protein 60 (HSP60, HSPD1) is a “chaperonin” that facilitates folding
2025-03-20 17 -
Selective Immortalization of Tumor-Specific T Cells to Establish Long-Term T-Cell Lines Maintaining
Antigen-specific T cells play a key role in cellular immune response against can
2025-03-20 13 -
Phosphatidylinositol Kinase Activity in Ovarian Cancer Cells
Phosphoinositides constitute less than 0.1% of total cellular lipids, yet accumu
2025-03-20 9 -
华人博士发明单个神经细胞及突触体内标识技术
斯坦福大学生物学系神经生物学博士李凌，将在近期的《PLoS ONE》杂志上发表文章，宣布发明了一种对单个神经元细胞及突触进行体内标识的技术，并利用此技术成功地
2025-03-20 9 -
Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunopre
Immunoprecipitation is one of the most commonly used techniques to study protein
2025-03-20 11 -
FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Fluorescence In Situ Hybridization (FISH) is a powerful and robust technique all
2025-03-20 11 -
Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay
Acquiring functional knowledge from protein-protein interaction studies often ne
2025-03-20 11 -
Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cell
Multiple drug resistance, mediated by the expression and activity of ABC-transpo
2025-03-20 9 -
Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies
Monoclonal antibodies (MAbs) have broad therapeutic applications in cancer thera
2025-03-20 10 -
Control of Mitotic Entry After DNA Damage in Drosophila
In the presence of DNA damage, cells delay the entry into mitosis, presumably to
2025-03-20 12
