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Isolation and Culture of Melanoma and Naevus Cells and Cell Lines
Recently developed methods are described for the culture of cells from primary h
2025-03-19 9 -
DNA Methylation in Pancreatic Cancer: Protocols for the Isolation of DNA and Bisulfite Modification
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive tumor and still remains
2025-03-19 11 -
Analysis of Genome-Wide DNA Methylation Profiles by BeadChip Technology
This chapter covers the genome-wide DNA methylation analysis using microarray pl
2025-03-19 10 -
Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences
Translocations and other rearrangements of the MLL gene at chromosome band 11q23
2025-03-19 12 -
DNA Methylation in Promoter Region as Biomarkers in Prostate Cancer
The prostate gland is the most common site of cancer and the second leading caus
2025-03-19 12 -
Digital Candidate Gene Approach (DigiCGA) for Identification of Cancer Genes
The candidate gene approach is one of the most commonly used methods for identif
2025-03-19 12 -
Microdissection and Extraction of DNA From Archival Tissue
Microdissection of cells with distinct histological features from tissue section
2025-03-19 10 -
Isolation of Inflammatory Cells from Human Tumours
Inflammatory cells are present in many tumours, and understanding their function
2025-03-19 10 -
Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth
It is becoming more and more clear that angiogenic mechanisms leading to structu
2025-03-19 10 -
Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography
Gelatin zymography is a simple yet powerful method to detect proteolytic enzymes
2025-03-19 11 -
Utility of Fine-Needle Aspiration Biopsy for Prospective Analysis of Patients Undergoing Therapy for
Many of the recent advancements in the area of tumor immunobiology have allowed
2025-03-19 9 -
Conformation-Sensitive Gel Electrophoresis for Detecting BRCA1 Mutations
Detection of mutations is of central importance in the study of genetic and mali
2025-03-19 11 -
Cancer Gene Therapy With Heat Shock Protein-65 Gene
Heat shock proteins (HSPs) are highly conserved proteins present in every living
2025-03-19 9 -
Angiogenesis, Metastasis, and Lung Cancer: An Overview
Tumor angiogenesis enables a pre-existing tumor to grow and metastasize. The ter
2025-03-19 8 -
Karyotyping Lymph Node Biopsies in Non-Hodgkins Lymphoma
Culture and transport methods are described that allow chromosomes to be obtaine
2025-03-19 12
