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COLD-PCR Enriches Low-Level Variant DNA Sequences and Increases the Sensitivity of Genetic Testing
Detection of low-level mutations is important for cancer biomarker and therapy t
2025-03-18 11 -
SAGE as a Strategy to Isolate Cancer-Related Genes
Serial Analysis of Gene Expression (SAGE) is a sequence-based approach that enab
2025-03-18 11 -
Models of Hormone Resistance In Vitro and In Vivo
Estrogen receptor (ER)-positive MCF-7 breast cancer cell lines can be used both
2025-03-18 10 -
Evaluation of Environmental and Personal Susceptibility Characteristics That Modify Genetic Risks
Study design in understanding gene—environment interaction plays a crucial role.
2025-03-18 11 -
Angiogenesis Assays
Angiogenesis—the formation of a vascular network—is essential for the support of
2025-03-18 11 -
Antisense Oligonucleotides as Research Tools
The use of antisense oligonucleotides as both research tools and therapeutic mol
2025-03-18 11 -
Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues
This chapter details methods used for analysis of DNA copy-number changes in bre
2025-03-18 10 -
Apoptosis: A Technical Overview
Physiological or programmed cell death plays an important role during a variety
2025-03-18 11 -
Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH)
Differential gene expression represents the entry point for comprehending comple
2025-03-18 15 -
Determination of the Catalytic Activities of mTOR and Other Members of the Phosphoinositide-3-Kinase
Members of the phosphoinositide-3-kinase-related kinase (PIKK) family, which inc
2025-03-18 11 -
Signal Transduction: A Technical Overview
Delineation of downstream signal transduction pathways is important for determin
2025-03-18 10 -
Real-Time RT-PCR (Taqman) of Tumor mRNA to Predict Sensitivity of Specimens to 5-Fluorouracil
5-Fluorouracil (5-FU) is still a key drug in the treatment of various kinds of a
2025-03-18 9 -
Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach
It has been shown that antisense oligodeoxynucleotide (ODN) treatments provide a
2025-03-18 14 -
Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse B
The study of key mechanisms and molecules involved in the regulation of hematopo
2025-03-18 12 -
Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate
The major mechanism of imatinib resistance for patients with chronic myeloid leu
2025-03-18 12
