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侧群干细胞（sp细胞）的分离与分选
准备工作： DF12 加入0.1%BSA，100ml,取10ml冰浴，40ml放入孵箱；其余的放入4度； DNase I 100*; PI：50ug/ml; H
2025-03-07 13 -
转铁蛋白(transferrin)
转铁蛋白是血液中一种主要的糖蛋白, 负责将肝组织(是铁贮藏的主要场所)的铁向其它组织的细胞运输。没有结合铁的转铁蛋白称作脱铁转铁蛋白(apotransferri
2025-03-07 10 -
内分泌信号(endocrine signaling)
由内分泌细胞合成并分泌到细胞外进行信号传导的分子称为内分泌信号。一般为激素类物质。这类信号分子通讯方式的距离最远，覆盖整个生物体。内分泌信号的激素有三种类型:蛋
2025-03-07 7 -
转胞吞作用(transcytosis)
转胞吞作用是一种特殊的内吞作用，受体和配体在内吞中并未作任何处理,只是经细胞内转运到相反的方向, 然后通过胞吐作用, 将内吞物释放到细胞外,这种内吞主要发生在极
2025-03-07 18 -
显微镜放射自显影样品的制备及观察
相关专题 显微放射自显影就是运用放射性标记化合物掺入生物样品，以及用核乳胶记录生物样品内放射性同位素释放核射线的技术。根据放射性标记化合物的性质和银粒的分布部
2025-03-07 19 -
Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells
Protein kinase A (PKA) activity is regulated by intracellular cyclic adenosine m
2025-03-07 14 -
Confirmation of the Presence of Drugs of Abuse in Urine
Urine screenings for drugs of abuse are performed for many reasons, two of the m
2025-03-07 11 -
小分子RNA(small RNA)
存在于真核生物细胞核和细胞质中，它们的长度为100到300个碱基(酵母中最长的约1000个碱基)。多的每个细胞中可含有105 ～106 个这种RNA分子，少的则
2025-03-07 13 -
Thin-Layer Gel Filtration: Determination of Protein Molecular Weights
For many years Sephadex gel filtration of proteins has been a valuable tool for
2025-03-07 14 -
Oocyte Extracts for the Study of Meiotic M-M Transition
In meiotic cell cycles, meiosis I (MI) is followed by meiosis II (Mil) without a
2025-03-07 10 -
胚胎干细胞培养操作规程
维持ES细胞处于未分化状态：ES细胞培养用含有ESGRO（白血病抑制因子）的高糖培养基来阻止细胞的分化。为细胞提供包被有0.1％明胶的平板作为粘附细胞的基质。建
2025-03-07 13 -
Rockland Dr.Moncada 分享： 如何获得更漂亮的流式结果
1. 如何降低背景 流式细胞实验优化过程中的一个关键步骤是试剂（抗体）的滴定。为了达到最佳效果，实验者必须确定抗原结合达到饱和所需抗体的最低量。这将帮助实验者提
2025-03-07 16 -
Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo
Hematopoietic stem and progenitor cells (HSPCs) are located in the bone marrow i
2025-03-07 12 -
In Situ Hybridization of Whole-Mount Embryos
Since the early analyses of gene expression in the Drosophila embryo (1 ), whole
2025-03-07 13 -
Autoradiography of 1-and 2-Adrenoceptors
Receptor autoradiography is derived from receptor binding techniques that were o
2025-03-07 15
