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  • MTS检测细胞生长

    MTS检测细胞生长

    1. 培养 IL-2 依赖细胞株 CTLL-2 细胞或 HT-2 细胞(检测 IL-2 ),或者 IL-3 依赖细胞株 FDC-P1 细胞或 FL5.12 细胞

    2025-03-07 9
  • Measurements of White Adipose Tissue Metabolism by Microdialysis Technique

    Measurements of White Adipose Tissue Metabolism by Microdialysis Technique

    The microdialysis method was introduced 25 yr ago to measure neurotransmitter co

    2025-03-07 11
  • Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis

    Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis

    Liquid chromatography (LC) can be combined with matrix-assisted laser-desorption

    2025-03-07 8
  • In Vitro Matrigel Angiogenesis Assays

    In Vitro Matrigel Angiogenesis Assays

    A variety of in vivo and in vitro methods have been used to study angiogenesis,

    2025-03-07 13
  • Locked Nucleic Acid Aptamers

    Locked Nucleic Acid Aptamers

    The aptamer technology has been introduced in the early 1990s. With this techniq

    2025-03-07 11
  • 核仁组织区(nucleolus organiting region,NOR)

    核仁组织区(nucleolus organiting region,NOR)

    参与形成核仁时的染色质区,核仁从核仁组织区部位产生,同时与该区紧密相连。具有核仁组织区的染色体称核仁染色体。核仁组织区定位在核仁染色体次缢痕部位。对人来说,在1

    2025-03-07 15
  • 原核融合(pronuclei fussion)

    原核融合(pronuclei fussion)

    精细胞的细胞核进入卵细胞质后,包装紧密的染色质开始解旋。精子的细胞核膜解体形成小的囊泡,并立即与精子细胞核脱离,形式没有核被膜的精子染色质,但很快形成新的核被膜

    2025-03-07 14
  • 分裂间期

    分裂间期

    真核细胞在细胞周期中,由上次细胞分裂结束到下次细胞分裂开始的持续时间。间期细胞进行旺盛的生物合成和生长,是细胞进入有丝分裂期的重要准备阶段。间期可划分为: G1

    2025-03-07 9
  • 细胞遗传学――原位杂交(ISH)

    细胞遗传学――原位杂交(ISH)

    In Situ Hybridization・ In Situ Hybridization (jsmith1@po-box.mcgill.ca )

    2025-03-07 11
  • Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay

    Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay

    Successful therapeutic angiogenesis requires an understanding of how the milieu

    2025-03-07 14
  • 激光显微细胞分离技术

    激光显微细胞分离技术

    在基础医学研究中涉及越来越多的如某一疾病状态组织中,多种基因或遗传变化,区别发展中的组织细胞群以及疾病状态组织细胞,将有助于了解疾病发生的分子机制。因此,在下一

    2025-03-07 10
  • Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damage

    Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damage

    Mammalian cells remove carcinogenic damage caused to DNA by ultraviolet (UV) lig

    2025-03-07 8
  • Keratinocyte Transient Transfections

    Keratinocyte Transient Transfections

    The goal of this chapter is to give the reader a concise and easy-to-follow guid

    2025-03-07 8
  • Quantitation of Sirolimus Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS)

    Quantitation of Sirolimus Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS)

    A multiple reaction monitoring positive ion HPLC method with tandem mass spectro

    2025-03-07 9
  • Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry

    Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry

    Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to an

    2025-03-07 10