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细胞生物基本方法：常规组织培养法
常规组织培养法 1 ）初代消化培养法 1. 准备：取各种已消毒的培养用品置于净化台面，紫外线消毒 20 分钟。开始工作前先洗手、 75% 酒精擦拭手至
2025-03-07 11 -
Well-Differentiated Human Airway Epithelial Cell Cultures
The airway epithelium occupies a critical environmental interface, protecting th
2025-03-07 13 -
Deep Sequencing of Small Chromatin-Associated RNA: Isolation and Library Preparation
Chromatin-associated RNA (caRNA) is a newly identified class of RNA molecules st
2025-03-07 11 -
多聚核糖体(polyribosomes)
在蛋白质合成过程中,同一条mRNA分子能够同多个核糖体结合，同时合成若干条蛋白质多肽链，结合在同一条mRNA上的核糖体就称为多聚核糖体(polysome 或po
2025-03-07 16 -
Over the past few years, the view that little or no cell turnover or replacement takes place within
TheADP-ribosylation factor proteins (ARFs) are ~20-kDa guanine-nucleotidebinding
2025-03-07 10 -
Cultures of Stem Cells of the Central Nervous System
Over the past few years, the view that little or no cell turnover or replacement
2025-03-07 13 -
CELL ADHESION
CELL ADHESION Procedure:1) Dissolve/dilute coating substrate in ddH2O at 4 C. A
2025-03-07 10 -
EB Differentiation
Aspirate medium from a 6-well plate and wash with PBS Ca++/Mg++ free. Incubate f
2025-03-07 16 -
胞内运输(intracellular transport)
真核生物细胞内膜结合细胞器与细胞内环境进行的物质交换。包括细胞核、线粒体、叶绿体、溶酶体、过氧化物酶体、高尔基体和内质网等与细胞内的物质交换。
2025-03-07 12 -
支原体检测：DNA萤光染色法
原理利用萤光染剂（bisbenzimide, Hoechst 33258）侦测支原体污染。此染剂会结合到DNA之Adenosine-Thymidine (A-T
2025-03-07 17 -
宿根福禄考组织培养及快繁技术
一、材料和方法1. 材料（1）材料取自当年带芽茎段为外植体，采自哈尔滨市农业科学院宿根花卉园。（2）培养基以MS 为基本培养基，附加不同的激素配比。启动、增殖培
2025-03-07 13 -
PAIR Technology: Exon-Specific RNA-Binding Protein Isolation in Live Cells
RNA-binding proteins (RBPs) are fundamental regulatory proteins for all forms of
2025-03-07 26 -
细菌培养倒平板的小技巧
相关专题 DNA连接与转化背景：在生物实验中相信大家都经常要倒平板，细菌 培养，分子生物学实验等等。医药企业中倒平板更是家常便饭，环境监测、水监测、微生物检查等
2025-03-07 13 -
In Situ Immunofluorescence Analysis: Immunofluorescence Microscopy
Immunofluorescence is one of the most widely used techniques to study the locali
2025-03-07 13 -
常用细胞凋亡检测方法（三）
三、线粒体膜势能的检测 线粒体在细胞凋亡的过程中起着枢纽作用，多种细胞凋亡刺激因子均可诱导不同的细胞发生凋亡，而线粒体跨膜电位DYmt的下降，被认为是细胞凋
2025-03-07 16
