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Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunopre
Immunoprecipitation is one of the most commonly used techniques to study protein
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FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Fluorescence In Situ Hybridization (FISH) is a powerful and robust technique all
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Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay
Acquiring functional knowledge from protein-protein interaction studies often ne
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Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cell
Multiple drug resistance, mediated by the expression and activity of ABC-transpo
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Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies
Monoclonal antibodies (MAbs) have broad therapeutic applications in cancer thera
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Control of Mitotic Entry After DNA Damage in Drosophila
In the presence of DNA damage, cells delay the entry into mitosis, presumably to
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Apoptotic Caspase Activation and Activity
Caspases are central to the execution of apoptosis. Their proteolytic activity i
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DNA-Mediated Gene Transfer
Transfection is the process by which exogenous DNA is transferred into eukaryoti
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Analyzing the Spindle Checkpoint in Yeast and Frogs
The spindle checkpoint is an evolutionarily conserved regulatory mechanism that
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Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGFα, and EGFR
Growth factors are polypeptides that induce cell mitogenicity, and thus play an
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Flow Cytometric Analysis of p53-Induced Apoptosis
The p53 protein is a key player in the cellular response to stress conditions. A
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Microdissection of Histologic Sections: Manual and Laser Capture Microdissection Techniques
The molecular analysis of human cancer is complicated by the difficulty in obtai
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Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection
Ovarian cancer remains a major health problem in the United States and most West
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干细胞和衰老的终结（第四部分）
“生命潜能：干细胞、克隆和再生”之Stem Cells and the End of Aging主持人：Thomas R.Cech, Ph.D.Presiden
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外源基因在真核细胞表达技术
本篇中主要介绍生化方法及物理方法（电穿孔法）一、生化方法1、磷酸钙介导的质粒DNA转染真核细胞（1）转染前24 h，通过胰酶消化收集细胞，用适当的完全培养基以1
