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蛋白激酶A (protein kinase A,PKA)
又称依赖于cAMP的蛋白激酶A (cyclic-AMP dependent protein kinase A),是一种结构最简单、生化特性最清楚的蛋白激酶。PK
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Lyophilization of Nonviral Gene Delivery Systems
This chapter presents a qualitative description of the freeze-drying process as
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Definitions and Criteria for Stem Cells
The working definition of a stem cell includes self-renewal and the ability to d
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Isolating primary B cells from human blood
1. start with freshly drawn human blood not older than 8 hours, treated with an
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线粒体膜势能检测细胞凋亡
线粒体在细胞凋亡的过程中起着枢纽作用,多种细胞凋亡刺激因子均可诱导不同的细胞发生凋亡,而线粒体跨膜电位的下降,被认为是细胞凋亡级联反应过程中最早发生的事件,它
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大肠杆菌杂交
相关专题 一、实验原理Lederberg和Tatum(1946)选用典型的大肠杆菌为材料,筛选营养缺陷型。利用双重和三重缺陷型的菌株,在简单的合成培养基上混合
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嘌呤霉素(puromycin)
嘌呤霉素是一种蛋白质合成抑制剂,它具有与tRNA分子末端类似的结构, 能够同氨基酸结合,代替氨酰化的tRNA同核糖体的A位点结合,并掺入到生长的肽链中。虽然嘌呤
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小鼠的R1胚胎干细胞系培养实验步骤
相关专题 以下是根据NIH老年病研究所 提供的英文的R1胚胎干细胞培养protocol整理而成。根据经验作部分修改。1、一般培养:保持胚胎干细胞 处于未分化状
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兔子白细胞介素10(IL-10)酶联免疫分析
兔子白细胞介素10 (IL-10) 酶联免疫 分析试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定兔子血清,血浆,细胞上清及相关液
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Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enrichi
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Induction of Tumor Cell Apoptosis and Chemosensitization by Antisense Strategies
Radiation and traditional chemotherapy kill both normal and tumor cells owing to
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细胞组分和细胞器――染色体
Chromosomal DNA Prep : cultured cells/tissue samples (Mike A Dyer) This protocol
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Intracellular Delivery of Nanoparticles with CPPs
Cell-penetrating peptides (CPPs), in particular TATp, have been widely used for
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红细胞裂解液的若干配制方法
配制试剂所需材料: 1. Ammonium Chloride 10x Lysing Concentrate Solution a) NH4Cl 80 g b)
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毕式酵母表达常见问题及回答
相关专题 1、问:我用毕式酵母表达一个27KD的蛋白,小量表达并做SDS-PAGE有一条类似三聚体的条带。用大量表达,表达上清用镍柱进行亲和层析 ,在FPLC