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Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans
The nematode Caenorhabditis elegans is an excellent model organism for studying
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Effects of Temperature Generated from the Holmium: YAG Laser on Human Osteoblasts in Monolayer Tissu
With the use of lasers for ablation purposes in spinal surgery, the tissue tempe
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Beta-gal staining of eukaryotic cells in vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols
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Assays of MSCs with Microarrays
The rapid development of microarray technology during the last decade has greatl
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Oligonucleotide FISH Probes
The use of highly specific synthetic oligonucleotide (ODN) probes, which have su
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Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional An
In this chapter, we explain different strategies to analyze the extracellular He
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动物细胞大规模微载体培养技术
相关专题 一、微载体 培养应用 此技术于1967年被用于动物细胞大规模培养。经过三十余年的发展，该技术目前已渐日趋完善和成熟，并广泛应用于生产疫苗、基因工程产
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Transforming chemically competent cells
Method Thaw TSS cells on ice. Add DNA, pipette gently to mix (1μl of prepped pla
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The Analysis of tRNA Import Into Mammalian Mitochondria
Ribonucleic acid (RNA) import into mitochondria occurs in a variety of organisms
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Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas Chromatography-Mass Spectrometry (G
Phencyclidine (PCP) is a cycloalkylamine and is classified as a dissociative ane
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Comparison of lysis methods
Comparison of lysis methods Sonicationmost efficient method of cell fractionatio
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Amniote Phylogenomics: Testing Evolutionary Hypotheses with BAC Library Scanning and Targeted Clone
Phylogenomics research integrating established principles of systematic biology
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Application of Adenoviral Vectors: Analysis of Eye Development
Recent technological advances in the generation of recombinant adenovirus vector
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Interspecific Chimeras: Transplantation of Neural Crest Between Mouse and Chick Embryos
Several labs have published data using tissue from genetically altered mouse emb
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细胞生物基本方法：冻存
冻存方法1． 细胞：选对数生长期细胞，收集细胞 24 小时前换液一次。2． 计数：按常规方法把细胞制成细胞悬液，计数，令细胞密度达 5 106 /ml ，离心
