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Isolation and Culture of Primary Human Hepatocytes
As our knowledge of the species differences in drug metabolism and drug-induced
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Expression of Recombinant Membrane-Type MMPs
Since the discovery of the membrane-type matrix metalloproteinases (MT-MMPs) whi
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Computational Prediction of RNA Structural Motifs Involved in Post-Transcriptional Regulatory Proces
mRNA molecules are tightly regulated, mostly through interactions with proteins
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Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease
A patient suspected of an inborn error of metabolism will commonly have urine or
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Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta
Abnormal proliferation of human vascular smooth muscle cells (hVSMCs) is a centr
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Purification Through Affinity Chromatography and Microsequencing
Production of monoclonal antibodies (MAbs) using adhesion-involved cell types as
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Local Application of Antisense for Prevention of Restenosis
In 1977, percutaneous transluminal coronary angioplasty (PTCA) was first introdu
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小泡运输(transport by vesicles)
蛋白质从内质网转运到高尔基体以及从高尔基体转运到溶酶体、分泌泡、细胞质膜、细胞外等则是由小泡介导的,这种小泡称为运输小泡(transport vesicles)
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前中期(prometaphase)
介于前期与中期之间的一个过渡期。前中期的主要特征是染色体剧烈地活动, 个别染色体剧烈地旋转、振荡、徘徊于两极之间。此时期的主要事件是纺锤体(spindle)的装
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The Measurement of Nitric Oxide and Its Metabolites in Biological Samples by Ozone-Based Chemilumine
A plethora of publications on techniques and methodologies for measuring nitric
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Protein Degradation Via the Proteosome
It is becoming apparent that an increase in the rate of protein degradation repr
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Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans
The nematode Caenorhabditis elegans is an excellent model organism for studying
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Effects of Temperature Generated from the Holmium: YAG Laser on Human Osteoblasts in Monolayer Tissu
With the use of lasers for ablation purposes in spinal surgery, the tissue tempe
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Beta-gal staining of eukaryotic cells in vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols
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Assays of MSCs with Microarrays
The rapid development of microarray technology during the last decade has greatl