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Primary Mouse Keratinocyte Culture
Mouse epidermal keratinocytes have traditionally been difficult to grow in vitro
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Gene Expression in Mouse Oocytes by RNA-Seq
Second-generation sequencers such as the Illumina GAIIx make possible the study
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Localization of mRNA in Vertebrate Axonal Compartments by In Situ Hybridization
The conclusive demonstration of RNA in vertebrate axons by in situ hybridization
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The In Vivo Evaluation of Active-Site TOR Inhibitors in Models of BCR-ABL+ Leukemia
Preclinical evaluation of candidate anticancer compounds requires appropriate an
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Analysis of Small Hsp Phosphorylation
Small heat-shock proteins (sHsps) are involved in diverse biological phenomena a
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Electrical Impedance Spectroscopy for Rapid and Noninvasive Analysis of Skin Electroporation
Transient disruption of skin’s barrier properties using high-voltage pulses invo
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Preparation of Segmented and Polarity Marked Microtubules
Segmented and polarity-marked microtubules are very useful for many different ty
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Chemotaxis in Neutrophil-Like HL-60 Cells
Asymmetric localization of intracellular proteins and signals directs movement d
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Immunolocalization in the Epidermis
Immunohistochemistry is an efficient means of localizing specific proteins to th
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In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping
Multicomponent RNA-protein complexes are essential for eukaryotic gene expressio
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Noncovalent Protein Interaction with Poly(ADP-Ribose)
Compared to most common posttranslational modifications of proteins, a peculiari
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Expression Profiling Using Quantitative Hybridization on Macroarrays
Expression studies performed on a genome scale have become very popular and prov
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Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammali
The control of gene function by the modulation of mRNA translation, stability, o
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Methylated Polyamines as Research Tools
Earlier unknown racemic β-methylspermidine (β-MeSpd) and γ-methylspermidine (γ-M
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Subcellular Fractionation of Adipocytes and 3T3-L1 Cells
For more than 30 yr, adipocytes have been an almost ideal model for the study of
