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Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells
It has been recently identified that cytokines and BMP-4 promote hematopoiesis f
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Multifluorescence Labeling and Colocalization Analyses
The fluorescence labeling technique is a method with a high degree of specificit
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Site-Directed Mutagenesis Using Positive Antibiotic Selection
A number protocols have been established for site-directed mutagenesis based on
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The Coculture Method to Examine Interactions Between Claudin Isoforms in Tight Junction-Free HEK293
The paracellular transport of water, ions, and small solutes is regulated by tig
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Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spi
The hair follicle is dynamic, cycling between growth (anagen), regression (catag
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Monitoring Patterns of Gene Expression in Dictyostelium by -galacotsidase Staining
Monitoring the spatial distribution of prespore and pstB cell types is a sensiti
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Skin Morphogenesis: Embryonic Chicken Skin Explant Cultures
Chicken skin development is an excellent model to study the mechanisms of morpho
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Labeling and Purification of RNA Synthesized by In Vitro Transcription
The problems of isolating sufficient quantities of rare RNAs for detailed bioche
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Neural Differentiation of Embryonic Stem Cells
Differentiation of pluripotent embryonic stem (ES) cells into specific lineages
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A rapid and highly sensitive method for somatic mutation profiling using the Sequenom MassARRAY® Sys
AbstractTumor genotyping can provide a useful guide to drive clinical trials, in
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Gene Transfer into Cystic Fibrosis Airway Epithelial Cells
Gene transfer into airway epithelial cells becomes a particularly motivating goa
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Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies
High quality DNA extractions developed for mammalian somatic cells are ineffecti
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Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival
The Rit subfamily of GTPases is a founding branch within the Ras family of small
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Protoplast Transformation of Filamentous Fungi
The protoplast method for the transformation of filamentous fungi is described i
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Purification of Peripheral Blood Natural Killer Cells
The ability to perform biological studies on natural killer (NK) cells requires
