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电位-门控通道(voltage-gated channels)
这类通道的构型变化依据细胞内外带电离子的状态,主要是通过膜电位的变化使其构型发生改变, 从而将“门”打开。在很多情况下, 门通道有其自己的关闭机制, 它能快速地
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“夸娥”因子的作用介绍
7月25日,复旦大学对外发布了一项基因研究领域里的重大成果,他们发现了破解基因“天书”的密码,这项具有“里程碑”意义的研究将在8月12日刊登在国际生物界权威杂志
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Inhibition of PI3K-Akt-mTOR Signaling in Glioblastoma by mTORC1/2 Inhibitors
Amplification of the gene encoding the epidermal growth factor receptor (EGFR) o
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Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand
Phosphorylation of histone H2AX on Ser 139 is a sensitive reporter of DNA damage
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In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Character
Of critical importance to many of the events underlying transcriptional control
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Revealing the Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray
In the past decade, the advent of microarray technologies has allowed functional
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Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
The interaction of drugs and dyes with nucleic acids, particularly when binding
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Quality Control Considerations
IntroductionQuality is important in all aspects of tissue culture since the qu
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Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs
The identification of protein binding partners often facilitates understanding o
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In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display
RNA-binding proteins (RNA-BPs) play an essential role in key processes in all li
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Human Embryonic Stem (ES) Cell Protocols――General notes on ES cell culture
hES media has a two week shelf life. hES cells should be cultured in 4, 6, 24, 4
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Endothelial wound healing (cell migration) assay
Description This is a simple assay that can be used in any cell culture lab setu
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Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues
Image analysis is vital for extracting quantitative information from biological
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Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent
Advances in stem therapy, scaffolds, and therapeutic biomolecules are accelerati
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Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications
Advances in our understanding of cardiomyocyte cell biology have been dependent