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Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells
Human Embryonic stem cells (hESCs) offer an invaluable tool for revealing human
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Purification of PCR Products from Agarose Gels for Direct Sequencing
The advent of direct sequencing of polymerase chain reaction (PCR) products has
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水稻根尖染色体标本制备
1.取材： 选取水稻种子，充分浸种后，将它们摆在铺有滤纸的培养皿内，在约28℃条件下发芽培养，当胚根长至1～1.5cm时，剪下备用。 2.预处理 压片法：采用0
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Phage Display of Peptides in Ligand Selection for Use in Affinity Chromatography
Large repertoires of peptides displayed on bacteriophage have been extensively u
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Ex Vivo Expansion of Haematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation
The efficient use of haematopoietic stem cells (HSC) for transplantation is ofte
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Sequencing of Antibodies
Several antireceptor monoclonal antibodies (MAbs) have been described to compete
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How do I decontaminate my tissue culture
When an irreplaceable culture becomes contaminated, researchers may attempt to e
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Peptide-Induced Fusion of Liposomes
Current experimental evidence suggests that the merging of two closely apposed l
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Modeling Spatial and Temporal Dynamics of Chemotactic Networks
When stimulated by chemoattractants, eukaryotic cells respond through a combinat
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Site-Specific Cleavage of Fusion Proteins
Where an affinity tag has served its purpose, it may become desirable to remove
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Preparation of Low Density, Collagenase-Digested Splenocytes (Hedrick Lab, UCSD Cancer Center)
1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/M
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A Practical Guide for using the AdEasy System
相关专题Tong-Chuan He, Kenneth W. Kinzler, and Bert Vogelstein Howard Hughes Medical
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Transfection of Osteoclasts and Osteoclast Precursors
Osteoclasts and their precursors have traditionally been considered difficult ce
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Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
The kidney is an extremely heterogeneous organ from a morphological and a functi
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Method: Reactivating Cell Lines and Cell Growth for DNA Preparation
urpose: Cell lines are reactivated and grown to a count of 1 x 108 cells. The c
