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Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes
The planar lipid bilayer technique is a powerful experimental approach for elect
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ERK-MAP Kinase Signaling in the Cytoplasm
ERK-MAPK is activated by dual phosphorylation of its activation loop TEY motif b
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Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics
The yeast Saccharomyces cerevisiae has become a valuable eukaryotic model organi
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Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors
Recent advances in cell biology have demonstrated the role of multiple signaling
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High Sensitivity Measurement of Estrone and Estradiol in Serum and Plasma Using LC-MS/MS
Measurement of low concentrations of estrogens, encountered in pre-pubertal chil
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Silencing in Yeast: Identification of Clr4 Targets
Efficient handling of multiple reactions is a crucial prerequisite for productiv
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Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature
In vivo preclinical assays are required to screen potential agents that target t
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Knockout Mice and Steroid Receptor Research
The development of techniques that allow defined alterations of the mammalian ge
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Ultrastructural Analysis of Cell Fusion in Yeast
The process of creating a single cell from two progenitor cells requires molecul
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Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry
Hydrogen exchange mass spectrometry (HX-MS) is an experimental technique that ca
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A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-cultures
MicroRNAs (miRNAs) are a class of small molecules, which regulate cellular funct
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Monitoring of Mycophenolate Acid in Serum or Plasma Using LC Tandem Mass Spectrometry
Therapeutic drug management of patients receiving mycophenolic mofetil or mycoph
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Analysis of Changes in Protein Level and Subcellular Localization During Cell Cycle Progression Usin
Methods are described here to monitor changes in protein level and subcellular l
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Purification of Recombinant Poly(ADP-Ribose) Polymerases
The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9
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de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination
In this protocol, we describe an approach in which two-dimensional electrophores
