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G-蛋白偶联受体(G-protein linked receptor)
配体与受体结合后激活相邻的G-蛋白, 被激活的G-蛋白又可激活或抑制一种产生特异第二信使的酶或离子通道,引起膜电位的变化。由于这种受体参与的信号转导作用要与GT
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Transfection of Adipocytes and Preparation of Nuclear Extracts
The uniqueness of the adipose cell type must be considered before choosing a pro
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Utility of Array CGH in Molecular Diagnosis of Mitochondrial Disorders
Array comparative genomic hybridization (aCGH) is a powerful clinical diagnostic
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Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resin
The strong interaction between streptavidin and biotin is one of the most common
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Simple Experimental and Spontaneous Metastasis Assays in Mice
Many steps of the metastatic cascade can be reproduced in simple in vitro assays
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Functional Genomics by Gene-Trapping in Embryonic Stem Cells
The pace of sequencing whole genomes by far exceeds our increase of knowledge on
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原生质体(potoplast)
脱去细胞壁的细胞叫原生质体, 是一生物工程学的概念。如植物细胞和细菌(或其它有细胞壁的细胞)通过酶解使细胞壁溶解而得到的具有质膜的原生质球状体。动物细胞就相当于
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Development of ATP-Competitive mTOR Inhibitors
The mammalian Target of Rapamycin (mTOR)-mediated signaling transduction pathway
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Detecting Expression Patterns of Wnt Pathway Components in Sea Urchin Embryos
The animal–vegetal (A–V) axis is a maternally established asymmetry that is pres
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Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes
The principle of subtraction hybridization protocols is the removal from one cel
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The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins
Biochemical assay of proteomic libraries derived from the Saccharomyces cerevisi
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Measuring Redox Changes In Vivo in Leaves: Prospects and Technical Challenges
In leaves, the functioning of many key proteins under conditions promoting oxida
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Quantitation of Benzodiazepines in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS
The benzodiazepine assay utilizes gas chromatography-mass spectrometry (GC-MS) f
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A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor
Changes in conformation are an important regulatory mechanism for a wide variety
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FISH Analysis of Human Pluripotent Stem Cells
Human pluripotent stem cells (PSCs) hold promise for treating a multitude of dis
