-
Nuclear Transfer in Nonhuman Primates
The nonhuman primate is a highly relevant model for the study of human diseases,
-
Nanosizing by Spatially Modulated Illumination (SMI) Microscopy and Applications to the Nucleus
In this chapter we present the method of spatially modulated illumination (SMI)
-
Imaging Plasma Membrane Proteins by Confocal Microscopy
Confocal microscopy has become a widely used method in the study of plasma membr
-
Homologous Gene Targeting to Study ECM Assembly
Genetically engineered mutant strains of mice have become invaluable too to deci
-
Assay for Protein Modification by Poly-ADP-Ribose In Vitro
The enzymatic function of poly(adenosine diphosphate (ADP)-ribose) polymerase (P
-
Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Supp
Over the last nine years, several quantitative polymerase chain reaction (QPCR)
-
Expression of EGF and HIV Envelope Glycoprotein
This chapter reviews the expression of two proteins that are naturally secreted:
-
Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry
Progression of cells through successive phases and checkpoints of the cell cycle
-
Extrachromosomal Assay for DNA Double-Strand Break Repair
DNA double-strand break (DSB) repair in mammalian cells has been demonstrated to
-
Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Indiv
In order to restore or to introduce a gene function integrating viral vector sys
-
Direct Sequencing of PCR Products Using Chemiluminescent Detection
The following protocol describes a method that can be used for the direct sequen
-
Live Imaging of LFA-1-Dependent T-Cell Motility and Stop Signals
T-cell motility is critical for leukocyte trafficking both in normal host defens
-
Computer-Assisted Interpretation of Triacylglycerols Mass Spectra
Triacylglycerols (TGs) are principal components of vegetable oils and animal fat
-
胶原(collagen)
胶原是细胞外最重要的水不溶性纤维蛋白, 是构成细胞外基质的骨架。胶原在细胞外基质中形成半晶体的纤维,给细胞提供抗张力和弹性,并在细胞的迁移和发育中起作用。胶原在
-
Group Migration and Signal Relay in Dictyostelium
The ability of cells to migrate directionally in gradients of chemoattractant is
