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Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
The nuclear–cytoplasmic distribution of ERK2 is regulated in response to various
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Use of Firefly Luciferase Activity Assays to Monitor Circadian Molecular Rhythms In Vivo and In Vitr
Circadian rhythms in metabolic, physiological, and behavioral processes are regu
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Investigation of KATP Channel Endocytosis by Immunofluorescence
Macroscopic ion channel currents (1 ) are a product of the channel open probabil
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Short-Term Chorionic Villi and Amniotic Fluid Cultures
Prenatal diagnosis of genetic disorders associated with specific biochemical, ch
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Internalization of Nucleoside Phosphates into Live Cells by Complex Formation with Different CPPs an
Nucleoside phosphates can bind to many functional proteins like G-proteins or ot
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Monitoring Sterol Uptake, Acetylation, and Export in Yeast
Sterols are essential lipid components of eukaryotic membranes. They are synthes
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Elastic Liposomes for Topical and Transdermal Drug Delivery
Elastic liposomes have been developed and evaluated as novel topical and transde
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New Opportunities for Immobilization of Enzymes
In this chapter, as a general introduction, we summarize our personal point of v
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Flow Cytometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoietic Stem and Progenitor
The hematopoietic system is highly proliferative in the bone marrow (BM) due to
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Immunofluorescence Analysis of Circadian Protein Dynamics in Cultured Mammalian Cells
The timing of both entry and permanence of core-clock proteins in the nucleus is
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Design of Experiments to Investigate Dynamic Cell Signaling Models
This chapter describes approaches to make use of dynamic models of cell signalin
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Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies
Measurement of DNA damage can be difficult if the levels of damage are small. Fo
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CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
The generation of dendritic cells (DCs) from monocytes and early progenitors in
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Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transducti
Mesenchymal stem cells (MSCs) are defined as self-renewing and multipotent cells
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MTT方法的使用步骤
1：接种细胞：用含10％胎小牛血清得培养液配成单个细胞悬液，以每孔 1000－10000个细胞接种到96孔板，每孔体积200ul. 2:培养细胞：同一般培养条件
