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糖原的显示―PAS反应（periodic acid schiff’s reaction）
目的要求 通过PAS反应，了解糖原显示的基本原理、方法以及糖原在细胞中的分布。 基本原理 糖原由D-葡萄糖的分支或直链组成，在肝和肌肉最丰富。过碘酸是一种强氧化
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Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC)
The culture and critical assessment of early human embryos during the first week
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Using 2D Crystals to Analyze the Structure of Membrane Proteins
Electron crystallography is a powerful technique for studying the structure and
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细胞培养基中常用维生素及其作用
相关专题 细胞培养基的配制维持细胞生长的生物活性物质，在细胞代谢中起调节及控制作用。在细胞培养 中，尽管血清是维生素重要来源， 但是许多培养基中添加了各种维生素
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Cloning of Potassium Channel Splice Variants from Tissues and Cells
Potassium channels display considerable functional diversity. Alternative pre-mR
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Immunohistochemistry of Xenopus Embryos
Immunohistochemistry is a very powerful technique for determining both the tissu
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Isolation of Hair Follicle Bulge Stem Cells from YFP-Expressing Reporter Mice
In this article we provide a method to isolate hair follicle stem cells that hav
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Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
Nucleotide excision repair (NER) is a particularly versatile pathway of DNA repa
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Functional Assays for Hematopoietic Stem Cell Self-Renewal
Stem cells are defined by the ability to self-renew. Specific functional assays
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Biomarkers of Cell Senescence Assessed by Imaging Cytometry
The characteristic features of senescent cells such as their “flattened” appeara
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PMEF FEEDER LAYER CONCENTRATION
Area/WellTotal AreaTotal CellsTotal VolConc/ml Flask cm2/cm25x104/cm2 96 Well0.
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Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue
Evidence is emerging that the mouse mammary epithelium is arranged as a hierarch
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ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/M
Epithelial-mesenchymal interactions during organogenesis are regulated by dynami
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The Intranuclear Environment
Many of the chapters in this volume are concerned with processes or structures i
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Culture Systems: Mineral Oil Overlay
Mineral oil overlay microdrop is commonly used during in vitro fertilization (IV
