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转染的时候能否用加血清的培养基
相关专题 总有一种转染方法适合你ayao0问:转染的时候可以用加血清的培养基吗前几天学习做细胞脂质体转染,看师姐是用的加血清的培养基,整个过程比以前的方法简单方
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Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis
Fluorescence Recovery After Photobleaching (FRAP) and Raster Image Correlation S
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主动运输
物质通过质膜进出细胞的方式之一。是质膜上载体蛋白消耗能量而协助物质逆浓度梯度运输的方式。主动运输和协助扩散虽然都是在载体蛋白协助下运输物质,但两者有两个主要区别
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实验室各种板子的用法比较
相关专题 细胞培养相关用品 细胞培养专题1.酶标板酶标板是一种配在酶标仪上做酶联免疫实验用的板子,常用的为96孔,主要是为了配合酶标仪所设计,另外还有48孔的,
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Mimicry Technology: Suppressing Small RNA Activity in Plants
Small RNA suppression constitutes one of the major difficulties for a full molec
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核膜
真核细胞围在细胞核外面的双层膜结构,是细胞核与细胞质之间的界膜。核膜由内外两层单位膜组成,每层膜厚约 6.5毫微米,两层膜间隙宽约 10~ 30毫微米,核膜外层
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细胞凋亡有什么特点?
相关专题 细胞的作用 细胞凋亡专题 生命活动的基本单位——细胞细胞凋亡 的特点:细胞变圆,染色质凝聚、分块,胞质皱缩。之后整个细胞通过发芽、起泡等方式形成一些球
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ARS 序列(autonomous replicating sequence)
即自主复制序列, 又叫复制起点序列(replication origin sequence)。是顺式作用元件的一种。这种序列是染色体正常起始复制所必需的。所有的
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Novagen公司推出无需IPTG的自动诱导大肠杆菌培养基
相关专题 细胞培养基的配制 大肠杆菌的基因工程最近Novagen 公司推出两种过夜培养自动诱导细胞表达的培养基系统,这两种培养基都可以最大程度上提高pET系列和
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In Vitro Stimulation and Detection of IFN Production in Human Plasmacytoid Dendritic Cells
Type-1 interferons (IFNs), including IFNα/β, are a family of cytokines produced
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Determination of Oxidized and Reduced CoQ10 and CoQ9 in Human Plasma/Serum Using HPLC-ECD
This chapter describes the use of reversed-phase HPLC with multichannel coulomet
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Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions
The bioluminescence resonance energy transfer (BRET) methodology allows for the
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Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Str
For recombinant expression of extracellular matrix (ECM) proteins or their indiv
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Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Mem
Epithelial cell polarity mediates many essential biological functions and pertur
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Endogenous Inhibitory Mechanisms and the Regulation of Platelet Function
The response of platelets to changes in the immediate environment is always a ba