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关于细胞培养基的几点讨论
相关专题培养细胞的完全培养基由基础培养基(如MEM)和添加剂(如血清或无血清培养用的某些确定的激素及生长因子)组成，培养基的配方一直在改进，其中包括抗生素和抗有
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Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques
Recently developed affinity purification methods have revolutionized our underst
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Resources for Genetic and Genomic Studies of Xenopus
The National Institutes of Health Xenopus Initiative is a concerted effort to in
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The Isolation of Nuclei and Nuclear Membranes from Rat Liver
Of all the organdies in rat hepatocytes, the nucleus is the largest and the most
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Functional Analysis of Cell Adhesion Molecules
Screening cDNA libraries by transient expression in mammalian cells has proven t
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Liposomal siRNA Delivery
With the recent discovery of small interfering RNA (siRNA), to silence the expre
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Single-Molecule Methods to Study Cell Adhesion Molecules
Single molecule techniques are used to characterize the biophysical properties o
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Detection and Measurement of Necrosis in Plants
Necrosis plays a fundamental role in plant physiology and pathology. When plants
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Fluorescence Methods in the Study of Small GTP-Binding Proteins
A great variety of small guanosine triphosphate (GTP)-binding proteins are known
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Antisense Strategies for Treatment of Heart Failure
In the mammalian heart, intracellular Ca2+ movements are tightly regulated at va
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Gene Expression and Methylation Patterns in Cloned Embryos
A considerable proportion of the offspring, in particular in ruminants and mouse
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Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine
The polyamines putrescine, spermidine, and spermine are essential for mammalian
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Cell Sorting By FACS
Currently, the Moflo instrument (Dakocytomation ) is used to sort cells at the A
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Identifying Myc Interactors
In this chapter, we discuss in detail two essential methods used to evaluate the
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Flow Cytometric Analysis of Drosophila Cells
Flow cytometry is a powerful technique that allows the researcher to measure flu
