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Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aort
We describe a method to derive cell lines and clones from cells of the murine mi
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Studying Nuclear Reprogramming with Cell Hybrids
Every cell in a mammal, other than odd exceptions (e.g. mature erythrocytes or l
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The Identification of Subcellular Fractions from Mammalian Cells
The chapters in the two volumes of Biomembrane Protocols are broadly devoted to
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Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
The method described in the following chapter utilizes a double thymidine block
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Methods for Studying Checkpoint Kinases Chk1
Attempts to passage through mitosis with unrepaired DNA damage or incompletely r
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Prespliceosome and Spliceosome Isolation and Analysis
Spliceosomes are multicomponent enzymes that remove introns from premessenger RN
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Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Cel
Nearly all hematopoietic cells in mammals derive from precursors that undergo mu
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Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software
Digital image analysis has been used to distinguish and quantify leaf color chan
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GeneCalling: Transcript Profiling Coupled to a Gene Database Query
We describe here the GeneCalling� method for the discovery of differentially exp
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Deep Sequencing of Small Chromatin-Associated RNA: Bioinformatic Analysis
Chromatin-associated RNA (caRNA) is a newly identified class of RNA stably linke
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Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope
Atomic force microscopy (AFM) can directly visualize single molecules in solutio
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Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines
In addition to regulating gene transcription, polyamines also potently modulate
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The Calvarial Injection Assay
This chapter describes the calvarial injection method, whereby the effect of a s
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Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
The skin is an important target for gene transfer because of its easy accessibil
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Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Cult
Repair of adult skeletal muscle depends on satellite cells, myogenic stem cells
