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In Situ Hybridization with Nonradioactive Probes
In situ hybridization takes advantage of the reaction between two complementary
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Analysis of Fungal Gene Expression by Real Time Quantitative PCR
The Real-Time quantitative PCR (qPCR) method has become central for the quantifi
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Detection of Protein Glutathionylation
Recent studies indicate that protein glutathionylation is an important regulator
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导肽(leading peptide)
又称转运肽(transit peptide)或导向序列(targeting sequence)，它是游离核糖体上合成的蛋白质的N-端信号。 导肽是新生蛋白N-端
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心肌细胞培养方法详述
配液：Hanks液、低糖DMEM液、高糖DMEM液、胰酶消化液、双抗液、碳酸氢钠液、盐酸液。 取鼠：鼠盆用棉铺好，取鼠。 准备：事先检查显微镜、磁力搅拌器及离心
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Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells
Embryonic stem (ES) cells are pluripotent stem cells derived from preimplantatio
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Analysis of Chromatin Composition of Repetitive Sequences: The ChIP-Chop Assay
Chromatin immunoprecipitation (ChIP) is a powerful method that allows to probe s
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Review of Molecular Mechanisms Involved in the Activation of the Nrf2-ARE Signaling Pathway by Chemo
Human exposures to environmental toxicants have been associated with etiology of
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Analysis of Fatty Acids by Gas Liquid Chromatography
The fatty acids (FA) of animal, plant, and microbial origin are predominantly un
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In Vivo Imaging of Tight Junctions Using ClaudinEGFP Transgenic Medaka
Tight junctions (TJs) function as a physiological barrier between epithelial and
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Culturing Chick Embryonic Cardiac Myocytes and Fibroblasts
Culturing Chick Embryonic Cardiac Myocytes and Fibroblasts Materials1. Culture m
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Visualization of PCP Defects in the Eye and Wing of Drosophila melanogaster
“ Tissue ” or “ planar ” polarity is a characteristic of many epithelial tissues
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Cdc28蛋白(Cdc 28 protein)
Cdc28蛋白是芽殖酵母CDC28基因编码的蛋白, 是第一个被分离的具有蛋白激酶活性的细胞周期蛋白。CDC28基因的功能相当于裂殖酵母的CDC2。CDC28基因
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Acute Behavioral Responses to Pheromones in C. elegans (Adult Behaviors: Attraction, Repulsion)
The pheromone drop test is a simple and robust behavioral assay to quantify acut
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Measurement of Caspase Activity: From Cell Populations to Individual Cells
Caspases are critical regulators of the apoptotic program, responsible for the h
