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Manipulating and Imaging the Early Xenopus laevis Embryo
Over the past half century, the Xenopus laevis embryo has become a popular model
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Derivation and Culture of Canine Embryonic Stem Cells
The derivation of canine embryonic stem cells (cESCs) represents a significant a
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Direct Sequencing of Differential Display PCR Products
The polymerase chain reaction (PCR)-based mRNA differential display (1 ) has bec
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Two-Hybrid Analysis of RasRaf Interactions
The yeast two-hybrid system ( 1 ) is a genetic method that enables the experimen
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Special Stains for Extracellular Matrix
Extracellular matrix and cells are the components of connective tissue. Extracel
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Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila
Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to an
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Manipulation of Gene Expression During Zebrafish Embryonic Development Using Transient Approaches
The rapid embryonic development and high fecundity of zebrafish contribute to th
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Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells
Many chronic liver diseases are life-threatening. When the liver loses the abili
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Restriction Enzymes
Restriction enzymes are endonucleases that recognize specific double-stranded DN
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Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins
The Ras-like low-molecular-weight guanosine triphosphate (GTP)-binding proteins
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Techniques for the Study of Apoptosis in Bone
There has been great interest in the identifying the mechanisms by which apoptos
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Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry
The organization of eukaryotic DNA is increasingly well understood at each extre
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Assessing the Involvement of Telomerase in Stem Cell Biology
Highly proliferative cells including stem cells and cancer cells express high le
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Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration
Altering the spatial relationships of embryonic or postembryonic tissues is one
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Identification and Characterization of Small GTPase-Associated Kinases
Rho-family signal-transduction cascades, which appear to be conserved in all euk
