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FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast
Yeasts represent powerful genetic model organisms, due in part to their tiny gen
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High-Resolution NMR of DNA and Drug-DNA Interactions
The advantage of NMR over most other spectroscopic techniques lies in the abilit
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Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells
Embryonic stem (ES) cells have a pluripotent ability to differentiate into a var
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Percent Labeled Mitosis Curve Analysis
The measurement of percent labeled mitoses (PLM, or alternatively, FLM, for frac
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Electroporation-Mediated Gene Transfer into Hepatocytes
The study of gene expression regulation relies on the introduction of foreign DN
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Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids
The first measurement of the transmembrane diffusion of phospholipids in membran
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细胞生物基本方法:神经组织细胞培养
神经组织细胞培养1. 获取脑组织后,先仔细剥除脑膜和血管等纤维成分,置入 Hanks 液中漂洗 1 ~ 2 次后,置于 30 ~ 50 倍的 Hanks 液中,
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Antisense-Mediated Inhibition of Protein Synthesis: Rational Drug Design, Pharmacokinetics, Intracer
There is hardly any class of drugs for which the term “ratronal drug design” is
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Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium
The retinal pigment epithelium (RPE) is a layer of multipotential cells of neura
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Protein Extraction for 2DE
Our protein extraction protocol for two-dimensional gel electrophoresis (2DE) wa
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Freezer and Cell Line Database
Very useful Microsoft Access database program (486kb) for managing your cell lin
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Assay of Phospholipase D Activity in Cell-Free Systems
Phospholipase D (PLD) enzymes are present in all animal and plant species and ha
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Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis
Bone, muscle, fat, and cartilage are differentiated adult tissues derived from e
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Flow Cytometry and Immunoselection of Human Stem Cells
Human hematopoietic stem cells (HSCs) can be obtained from a variety of hematopo
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Reprogramming of B Cells
Cellular reprogramming is an interplay between the original starting cell’s plas