-
Adipogenic Differentiation of Human Induced Pluripotent Stem Cells
Human induced pluripotent stem (iPS) cells are promising sources of disease mode
-
Frster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells
Understanding the molecular mechanisms of protein–protein interactions at the ce
-
Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay
The procedure described here, the PCR poly(A) test (PAT), allows the analysis of
-
The Isolation of Membranes from Bacteria
Perhaps the most striking feature of bacterial membranes is their multifunctiona
-
G带(Giemsa-banding)
将染色体制片经盐溶液、胰酶或碱处理, 再用吉母萨染料染色, 在光镜下进行检查, 见到特征性的带。一般富含AT碱基的DNA区段表现为暗带。此法可制成永久性的标本。
-
Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
It is now well understood that G protein-coupled receptor (GPCR)-mediated cell s
-
Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence
The budding yeast Saccharomyces cerevisiae has become a laboratory favorite for
-
Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells
Differentiated cell types derived from human embryonic stem cells (hESCs) may se
-
Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys
One of the target diseases of cell-replacement therapy is Parkinson’s disease. C
-
Immunoblotting 2DE Membranes
Two-dimensional electrophoresis (2DE) immunoblotting combines the resolving powe
-
Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease
The modification of cell surface proteins by plasma membrane and soluble proteas
-
同源异型基因（homeotic gene）
一类含有同源框的基因。在胚胎发育中的表达水平对于组织和器官的形成具有重要的调控作用。该类基因的突变，就会在胚胎发育过程中导致某一器官异位生长，即本来应该形成的正
-
Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissue
Claudins are integral proteins of the TJ. Each epithelia in the organism express
-
Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors
The yeast two-hybrid system, originally developed by Fields and Song (1 ), is a
-
Continuous-Flow Electrophoresis: Application to the Isolation of Lysosomes and Endosomes
Continuous-flow electrophoresis (CFE), which separates particles on the basis of
