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Expression of Epitope-Tagged Proteins in Plants
Although immunoelectron microscopy is a powerful tool for visualizing the subcel
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Cell Sorting of Young and Senescent Cells
Cellular senescence is the irreversible loss of proliferative potential and is a
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Assaying Lipid Phosphate Phosphatase Activities
Lipid phosphate molecules such as phosphatidate, lysophosphatidate, and diacylgl
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Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays
The PDZ domain is a protein-protein interaction module that interacts with a C-t
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Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time
The directed migration of cells (chemotaxis) occurs not only during wound healin
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Measurements of Active TGF Generated by Culture Cells
The transforming growth factors-β (TGF-βs) constitute a family of potent regulat
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Membrane Translocation Assayed by Fluorescence Spectroscopy
Assessing the ability of biomolecules or drugs to overcome lipid membranes in a
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单管(singlet)
是以单支存在的微管,大部分细胞质微管是单管微管, 它在低温、Ca2 和秋水仙素作用下容易解聚, 属于不稳定微管。虽然绝大多数单管是由13根原纤维组成的一个管状结
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Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells
Electroporation has been frequently adopted as a means to transfect primary cell
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Mammalian Fertilization Is Dependent on Multiple Membrane Fusion Events*
Successful completion of fertilization in mammals is dependent on three membrane
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Modeling Brain Pathologies Using Neural Stem Cells
The study of the mechanisms underlying neurodegenerative diseases is a highly de
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Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation
Dendritic cells (DCs) are a heterogenous population of professional antigen-pres
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G Protein-Coupled Receptor Dimerization and Signaling
G protein-coupled receptors are involved in the regulation of many aspects of no
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Pheromones in the Fruit Fly
The identification of pheromones (chemical communication cues) is critical to ou
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Mobilization of Hematopoietic Stem Cells by Depleting Bone Marrow Macrophages
An important factor contributing to hematopoietic stem cell (HSC) mobilization i
