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Immunohistochemistry of MMPs and TIMPs
Immunohistochemical techniques are a convenient method to identify the cells res
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Real-Time Analysis of Integrin-Dependent Transendothelial Migration and Integrin-Independent Interst
The role of integrins in leukocyte migration across endothelial barriers is wide
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Three-Dimensional Human Tissue Models of Wounded Skin
Human skin equivalents (HSEs) are in vitro tissues in which a fully differentiat
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Microscopic Computed Tomography-Based Virtual Histology of Embryos
Advances in imaging technologies and computational capabilities have made possib
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Intracellular Staining Protocol
1. Fix cells - Add16% formaldehyde directly into culture medium to obtain a fina
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Transgenic Mouse Models of Craniofacial Disorders
Disorders affecting craniofacial development represent a large fraction of birth
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Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice
Live imaging is a powerful approach to understanding the dynamic processes that
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High Proliferative Potential Colony Forming Cells
High proliferative potential colony forming cells (HPP-CFC) in mouse bone marrow
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A Human Cell Extract-Based Assay for the Activation of ATM and ATR Checkpoint Kinases
The Ataxia telangiectasia -mutated (ATM) and the ATM-Rad3-related (ATR) kinases
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Organ Cultures and Kidney-Capsule Grafting of Tooth Germs
The study of organogenesis allows investigation of a variety of basic biological
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The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells
The Ipl1/Aurora family of protein kinases are required for accurate chromosome s
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Liposomes for Drug Delivery to Mitochondria
Efficacy of therapeutically active drugs known to act on intracellular targets c
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Demonstration of Mycoplasma Contamination in Cell Cultures by a Mycoplasma Group- Specific Polymeras
Cell cultures are widely used in both medical and biotechnical research centers,
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关卡(check point)
严格地监视着细胞周期事件的发生、发展过程是否严格按程序进行控制点称为关卡。如酵母细胞在DNA合成开始前的启动点(start)、哺乳类细胞周期的G1期R点或限制点
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Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yea
A novel protein expression system in budding yeast is described which has been u