-
Minimization of Proteins by Random Fragmentation and Selection
Protein-protein interactions are involved in most biological processes and are a
-
Cytological Analysis of Meiosis in Fixed Drosophila Ovaries
Methods are described to analyze two different parts of the Drosophila ovary, wh
-
Isolation and Purification of Plant Nucleic Acids: Genomic and Chloroplast DNA
The use of molecular protocols has expanded into virtually all branches of plant
-
Hoechst 33342/PI双染色法
1.悬浮生长的细胞在培养状态下加入Heochst 33342,终浓度为1μg/ml;帖壁生长的细胞用含有0.02%EDTA的0.25%胰蛋白酶消化成单细胞悬液,
-
体内肝损伤模型(酒精)
一、材料 纯系SD雄性大鼠,体重180g~200 g,由浙江大学医学院实验动物中心提供。食用酒精选用北京酿酒总厂出品的56度红星二锅头。 二、方法 将大鼠随机分
-
293T细胞的一点培养心得
相关专题 293细胞293T细胞差点被我养死,幸好我又将它起死回生,一点心得。细胞传代:当细胞在细胞培养 瓶内长满达到80%时就要传代,一传二,24小时后再传代
-
Adrenal chromaffin granule (chromaffin vesicle) preparation
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This pr
-
人(ASD)酶联免疫分析
人 (ASD) 酶联免疫分析试剂盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中雄烯二酮(ASD) 的含量。实
-
Lacrimal Experimental Protocol (4/4/94)
Lacrimal Experimental Protocol (4/4/94)Day Before:1. Prepare (6) 50 ml conicals
-
PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Ano
Six morphologically indistinguishable but genetically and behaviorally distinct
-
Enrichment of PBMCs with monocytes (The Science Advisory Board)
Description This protocol is used in our lab to reduce the costs of the cell sor
-
胚胎干细胞培养标准化操作规程
目录 一、细胞 二、一般培养-保持胚胎干细胞处于未分化状态 培养基 细胞复苏 冻存细胞 明胶包被 细胞传代 三、体外分化 培养基 包被有多聚鸟氨酸/纤维结合蛋白
-
Production of Neurospheres from CNS Tissue
The relatively recent discovery of persistent adult neurogenesis has led to the
-
Isopycnic Centrifugation of DNA: Extraction and Fractionation of Melon Satellite DNA by Buoyant Dens
In order to study the organization and properties of DNA, it is necessary to ext
-
Immobilization of Enzymes on Ethynyl-Modified Electrodes via Click Chemistry
This paper describes a novel, simple, and versatile protocol for covalent immobi