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Bioinformatic Identification of Plant Peptides
Plant peptides play a number of important roles in defence, development and many
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Epidermal Cell Analysis by RT-PCR
Reverse transcription polymerase chain reaction is used for the semiquantitative
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Assessing Mitochondrial Potential, Calcium, and Redox State in Isolated Mammalian Cells Using Confoc
Mitochondria play a vital role in the regulation of intracellular calcium dynami
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Current Thoughts on the Therapeutic Potential of Stem Cell
Stem cells are considered as potential therapy for inflammatory disorders, tissu
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Murine Nonvolatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1
Our search for a substance recognized by the vomeronasal neurons revealed that t
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Determination of Hydroxyurea in Serum or Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)
Hydroxyurea is an antineoplastic drug, which is also widely used in the treatmen
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Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus
One of the major obstacles in generating induced pluripotent stem cells for rese
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Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomi
Xenopus oocytes, eggs, and embryos serve as an ideal model system to study sever
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Cryopreservation of Mammalian Embryos: Slow Cooling
The benefits of banking mammalian embryos for long-term storage have long been r
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Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cell
We describe here the isolation and maintenance of pluripotent embryonic stem (ES
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Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pe
The zona pellucida is an extracellular glycoprotein coat that surrounds the oocy
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Minimization of Proteins by Random Fragmentation and Selection
Protein-protein interactions are involved in most biological processes and are a
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Cytological Analysis of Meiosis in Fixed Drosophila Ovaries
Methods are described to analyze two different parts of the Drosophila ovary, wh
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Isolation and Purification of Plant Nucleic Acids: Genomic and Chloroplast DNA
The use of molecular protocols has expanded into virtually all branches of plant
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Hoechst 33342/PI双染色法
1．悬浮生长的细胞在培养状态下加入Heochst 33342，终浓度为1μg/ml；帖壁生长的细胞用含有0.02％EDTA的0.25％胰蛋白酶消化成单细胞悬液，
