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Oxidative Damage in Huntington’s Disease
Huntington’s disease is a hereditary neurodegenerative disorder, characterized b
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cRNA Probes: Comparison of Isotopic and Nonisotopic Detection Methods
Since the first description of the technique of in situ hybridization to detect
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New Protocol for Lentiviral Vector Mass Production
Multiplasmid transient transfection is the most widely used technique for the ge
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Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation
The partitioning of secretory and membrane protein-encoding mRNAs to the endopla
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The Genome of Dictyostelium discoideum
The Dictyostelium discoideum genome has been sequenced, assembled and annotated
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Methods for Assessing Effects of Wnt/-Catenin Signaling in Senescence of Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) represent a main population of stem cells and can
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Imaging Cells Within 3D Cell-Derived Matrix
Much of what is known about the mechanisms regulating cell adhesion and migratio
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Purification of Proteins Using Displacement Chromatography
Displacement chromatography has several advantages over the nonlinear elution te
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Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Neural stem cells are defined as cells that either gives rise to or derives from
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Site-Directed Mutagenesis to Study the StructureFunction Relationships of Ion Channels
Ion channels mediate a wide variety of physiological processes by forming small
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Preparation of Immobilized Subunits of a Multisubunit Enzyme
It is well established that many intracellular enzymes are oligomeric with dimer
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MTT Cell Proliferation Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first
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Gene Expression Analyzed by Ribonuclease Protection Assay
The ribonuclease (RNase) protection assay provides a highly sensitive method for
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Methods for Gene Expression Profiling in Dermatology Research Using DermArray Nylon Filter DNA Micro
Here we present methods of gene expression profiling using nylon filter deoxyrib
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Serial Section Immunoelectron Microscopy of Algal Cells
Electron microscopy when combined with immunogold labeling provides a 2D image o
