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DNA染色法检测支原体操作步骤
一、实验准备1、CO2培养箱、无菌小爬片。无菌培养皿、不含抗生素的完全培养液2、二苯甲酰胺荧光染料浓缩液(50μg/ml)。称取5mg二苯甲酰胺荧光染料加入10
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Dot blot斑点杂交实验原理与方法
斑点杂交(Dot blot)是将被检标本点到膜上,烘烤固定。这种方法耗时短,可做半定量分析。一张膜上可同时检测多个样品,为使点样准确方便,市售有多种多管吸印仪(
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RNA Dot Blot斑点杂交实操步骤
一、RNA乙酰化Dot Blot实操步骤1、实验准备仪器准备:超微量核酸分析仪、紫外交联仪、水平脱色摇床、化学发光仪、超净工作台、微量移液器、电热恒温鼓风干燥箱
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淋巴细胞亚群检测实验原理与介绍
首先,咱们先来看看淋巴细胞亚群检测的实验原理:流式利用不同荧光物质标记的单克隆抗体,与待测成分作用,然后上流式细胞仪检测待测细胞。待测细胞随流动室内的流动鞘液排
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Th淋巴细胞亚群检测步骤
细胞亚群的检测方法种类繁多,大多都是根据细胞表面抗原及其抗体的特异性反应设计的。随着单克隆抗体的问世及各种检测方法的建立,淋巴细胞亚群检测已经在临床上得到广泛应
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什么是粒细胞?
粒细胞是胞质中有特殊颗粒的白细胞。白细胞根据形态差异可分为颗粒和无颗粒两大类,颗粒白细胞(粒细胞)中含有特殊染色颗粒,用瑞氏染料染色可分辨出三种颗粒白细胞即中性
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粒细胞检测实验原理与流程
粒细胞检测最常用的方法是流式检测,其实验原理是流式利用不同荧光物质标记的单克隆抗体,与待测成分作用,然后上流式细胞仪检测待测细胞。待测细胞随流动室内的流动鞘液排
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什么巨噬细胞?
1、巨噬细胞巨噬细胞是一种位于组织内的白血球,源自单核细胞,而单核细胞又来源于骨髓中的前体细胞。巨噬细胞和单核细胞皆为吞噬细胞,在脊椎动物体内参与非特异性防卫(
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Isolation, Characterization, and Culture of Normal Human Intestinal Crypt and Villus Cells
The intestinal epithelium is a highly dynamic tissue undergoing constant and rap
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Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells
The stimulation of inositol phospholipid metabolism via phospholipase C (PLC) is
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Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cas
Measuring the effects of sphingosine-1-phosphate (S1P) receptor modulators on hu
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Molecular Cytogenetics: The Standard FISH and PRINS Procedure
In this chapter, the two basic principal techniques of molecular cytogenetics ar
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Molecular Links Between Autophagy and Apoptosis
Macroautophagy (herein referred to as autophagy) contributes to the control of l
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Live Imaging of Primitive Endoderm Precursors in the Mouse Blastocyst
The separation of two populations of cells—primitive endoderm and epiblast—withi
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Identification of Novel Genes Involved in Adipose Differentiation by Differential Display
In vertebrate animals, adipose tissue functions primarily to store excess energy