-
Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors
The central nervous system (CNS) is composed of multiple cell types formed throu
-
Derivation and Maintenance of Human Embryonic Stem Cells
Since their derivation in 1998, human embryonic stem cells (hESCs) have been the
-
Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic
This chapter describes a detailed protocol for deriving mouse embryonic stem (ES
-
Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells
Limited understanding of the cell biology of the breast and breast cancer hamper
-
Peptide Structure Determination by NMR
The difference between peptides and proteins (the subject of Chapter 2 ) is that
-
A first step in defining the function of a novel gene is to determine its interactions with other ge
The difference between peptides and proteins (the subject of Chapter 2 ) is that
-
Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
A first step in defining the function of a novel gene is to determine its intera
-
Human Embryonic Stem (ES) Cell Protocols――Splitting Human ES cells on MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 C in a wa
-
Vascular Progenitor Cell Mobilization
Blood vessel formation plays a key role in both physiologic and pathologic tissu
-
Cytochemical Probes of Cycling and Quiescent Cells Applicable to Flow Cytometry
For convenience the term quiescent cells will be used in this chapter to define
-
Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization
Medical applications of enzymes are found in various areas, such as diagnosis, p
-
Quantitative Polymerase Chain Reaction
Quantitative PCR (qPCR) has entered widespread use with the increasing availabil
-
Chromosome Spreading and Immunofluorescence Methods in Saccharomyes cerevisiae
Visualization of meiotic chromosomes in the model organism S. cerevisiae has bec
-
Fluorescence-Activated Cell Sorting Analysis of Mitochondrial Content, Membrane Potential, and Matri
Fluorescence-activated cell sorting (FACS) permits specific biologic parameters
-
Glycosylation Profiling of Heterologous Proteins
The methylotrophic yeast Pichia pastoris has been widely exploited for its high-
