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Mechanical Properties of Bone Ex Vivo
The primary functions of bone are to do with support and protection – mechanical
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Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis
Identification and characterization of proteins are ultimately the goal in prote
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Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Synchronized populations of large numbers of cells can be obtained by centrifuga
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Freezing and Thawing Cultured Cells
Freezing Cells: 1. Trypsinize cells and harvest in the normal way. 2. Count a 20
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The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells
In this chapter, we describe a method to isolate and to expand multipotent adipo
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In Vitro Culture of Rodent Embryos During the Early Postimplantation Period
Mammalian embryos grow within the uterus. This inaccessibility makes mammalian e
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Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
Lentiviral vectors based upon human immunodeficiency type I (HIV) are increasing
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Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases
G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs) are tra
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Imaging Ca2+ Signals in Xenopus Oocytes
Xenopus oocytes have become a favored preparation in which to study the spatiote
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Identification of MetaboliteRiboswitch Interactions Using Nucleotide Analog Interference Mapping and
Riboswitches are RNA elements capable of modulating gene expression through inte
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Reactive Oxygen Species and Upregulation of NADPH Oxidases in Mechanotransduction of Embryonic Stem
Deciphering the differentiation pathway of embryonic stem (ES) cells is a challe
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In Vitro Excision Repair Assay in Schizosaccharomyces pombe
The measurement of DNA excision repair activity in vitro, originally developed b
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Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and T
Substantial progress has been made in somatic cell reprogramming through ectopic
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Subculturing Cells
Subculturing Cells Materials1. Medium with supplements (serum, glutamine, antibi
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Signal Transduction Inhibitors in Cellular Function
Signal transduction pathways mediate cell-cell interactions and integrate signal
