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Isolation, Expansion, and Characterization of Human Islet-Derived Progenitor Cells
Islet transplantation is a widely accepted and practiced cell replacement therap
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Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Open
This chapter describes a method of assaying rubidium (Rb+ ) efflux as a measure
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ECM Degradation Assays for Analyzing Local Cell Invasion
Proteolytic degradation of extracellular matrix (ECM) is a critical step during
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Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis
Blue native gel electrophoresis (BNGE) is a powerful tool for analyzing native p
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Managing Apheresis Complications During the Hematopoietic Stem Cell Collection
Adverse reactions may occur during the Hematopoietic Progenitor Cells via Aphere
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Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)
The functional and molecular characterization of mouse epidermal stem cells (SC)
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A Historical Perspective of the EGF Receptor and Related Systems
Since the isolation of epidermal growth factor (EGF) from mouse submaxillary gla
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Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-T
Membrane proteomic analysis is of considerable interest due to the role of recep
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核纤层(lamina)
在与核质相邻的核膜内表面有一层厚30～160nm 网络状蛋白质, 叫核纤层, 对核被膜起支撑作用。核纤层由3种分子量为6～7万道尔顿的多肽亚单位α、β、γ所组成
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磷脂交换蛋白(phospholipid exchang proteins, PEP)
PEP是一种水溶性的载体蛋白,可以在不同的膜结合细胞器之间转移磷脂。转移的过程是: PEP首先与磷脂分子结合, 形成水溶性的复合物进入细胞质基质中, 通过自由扩
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Quantitative X-ray Imaging of Rodent Bone by Faxitron
This chapter describes the use of digital micro-radiography with the Faxitron ma
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Mechanical Properties of Bone Ex Vivo
The primary functions of bone are to do with support and protection – mechanical
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Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis
Identification and characterization of proteins are ultimately the goal in prote
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Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Synchronized populations of large numbers of cells can be obtained by centrifuga
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Freezing and Thawing Cultured Cells
Freezing Cells: 1. Trypsinize cells and harvest in the normal way. 2. Count a 20
