-
Screening Expression Libraries with Solution-Based Assay
To date, the most common approach to screening an expression library for an RNA-
-
Ras GTPases Are Both Regulators and Effectors of Redox Agents
Redox agents have been historically considered pathological agents which can rea
-
Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies
Sea urchins have long been used to study morphogenesis and cell fate specificati
-
停靠蛋白(docking protein, DP)
即SRP在内质网膜上的受体蛋白,它能够与结合有信号序列的SRP牢牢地结合,使正在合成蛋白质的核糖体停靠到内质网上来。停靠蛋白含有两个亚基，一个亚基暴露于细胞质的
-
Laboratory Guidelines for Animal Care
Animal research is a controversial subject because of the ethical and moral impl
-
Differentiate ES cells into cystic embryoid bodies
Differentiate ES cells into cystic embryoid bodiesDay -1: Pass ES cells at norma
-
Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo
The use of ionizing radiation as a therapeutic agent has been recognized for alm
-
Apoptosis: Miniassay
1) Aliquot 5 X 106 total cells in 5-8 ml 2% media. 2) Add 30 µl 3H-thymidine. 3)
-
Senescence Regulation by the p53 Protein Family
p53, a guardian of the genome, exerts its tumor suppression activity by regulati
-
Molecular Profiling of the Epidermis: A Proteomics Approach
Mouse skin has been used extensively as a model system to study the development
-
Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies
The advent of the “GFP technology” together with advances in digital imaging and
-
Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells
Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated
-
Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells
The integrity, or barrier function, of the intestinal epithelium is of paramount
-
Screening for Inhibitors of Microtubule-Associated Motor Proteins
The mitotic spindle is an important target for cancer chemotherapy. The main pro
-
Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis
The hallmark of hematopoietic stem and progenitor cells (HSPCs) is their motilit
