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圆球体(spherosome)
植物细胞中由一层单位膜包裹的含有细微结构的球形颗粒,直径为0.5~1μm,内含酸性水解酶,相当于动物细胞的溶酶体。与动物细胞的溶酶体不同,圆球体能够被脂溶性的染
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FISH Methods in Cytogenetic Studies
This chapter describes the various methods derived from the protocol of standard
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Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells
The use and study of neural stem cells (NSCs) both in vitro and in vivo has incr
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Sponge Implant Model of Angiogenesis
The process of capillary growth, angiogenesis, is an integral part of wound heal
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Microtubule Spindowns for Visual Analysis
Microtubule spindowns for visual analysis can be performed on single microtubule
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The Measurement of Cyclic AMP Levels in Biological Preparations
The generation of cyclic AMP (cAMP) is probably the most scrutinized of signal t
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Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches
Molecular chaperones function in a range of protein homeostatic events, includin
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Studying Mechanosensitive Ion Channels Using Liposomes
Mechanosensitive (MS) ion channels are the primary molecular transducers of mech
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Genetic-Engineered Models of Skeletal Diseases II: Targeting Mutations into Transgenic Mice Chondroc
The manipulation of mice genome is a powerful technique to analyze biological pr
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血影蛋白(spectrin)
又称收缩蛋白,是红细胞膜骨架的主要成份,但不是红细胞膜蛋白的成份,约占膜提取蛋白的30%。血影蛋白属红细胞的膜下蛋白,这种蛋白是一种长的、可伸缩的纤维状蛋白,长
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PACE Analysis of RNA-Peptide Interactions
The PACE assay is a relatively new addition to the arsenal of techniques used to
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分泌(secretion)
细胞内的分子排出或挤出到周围介质中。分泌物包括离子、酶、激素、糖蛋白、液体、无机盐等。分泌的主要形式如下。 局部分泌(merocrine) 或称外分泌(exo
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Generation and Characterization of Epiblast Stem Cells from Blastocyst-Stage Mouse Embryos
Mouse epiblast stem cells (EpiSCs) are pluripotent embryonic cells that can be u
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Goat Embryonic Stem-Like Cell Derivation and Characterization
Embryonic stem (ES) cells are derived from the inner cell masses of preimplantat
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Measurement of Autophagy in Cells and Tissues
Two major proteolysis systems, the ubiquitin-proteasome system, and the autophag