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Flow Sorting Fibroblasts with GFP and P.I.
Protocol Wash cells with PBS and trypsinize to a single cell suspension. Count a
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Sequencing PCR Products Cloned into M13 Vectors
The polymerase chain reaction (PCR) facilitates the rapid in vitro amplification
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基质(ground substance)
细胞外基质的物理性质主要受细胞外基质中蛋白聚糖所携带的多糖基团的影响,蛋白聚糖是由糖胺聚糖(glycosaminoglycans, GAG)以共价的形式同线性多
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大批量生产干细胞培养基的具体细节
相关专题 细胞培养基的配制杨等使用的bioreactor实际上是一种组织生长设备(tissue-growing device),能够用于培养成人干细胞 ,并且能
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Proteolytic Enzymes for Peptide Production
There are three main reasons why a protein chemist might wish to cleave a protei
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Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts
Mammalian spermatogenesis is a highly organized process of cell division and dif
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Assay for Neuronal Cell Migration
Chemoattractant-induced neuronal migration can be directly and quantitatively ev
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配体(ligand)
同锚定蛋白结合的任何分子都称为配体。在受体介导的内吞中, 与细胞质膜受体蛋白结合, 最后被吞入细胞的即是配体。根据配体的性质以及被细胞内吞后的作用, 将配体分为
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细胞介导细胞毒作用检测法5:用MTT检测法测定CMC
用 MTT 检测法测定 CMC 1. Fen 细胞培养于含 10 %小牛血清的 RPMI-1640 培养液中,在 25cm2 培养瓶中生长到接近汇合。用含 0.
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大鼠免疫球蛋白E(IgE)酶联免疫分析(ELISA)
大鼠 免疫球蛋白 E(IgE) 酶联免疫分析( ELISA )试剂盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体
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微球体体系
微球体体系包括细胞核内的染色质、染色体和核仁,以及细胞质中的核蛋白体,它们有特别密切的结构和功能的内在联系:从化学成份而言,它们都是核蛋白;核蛋白体虽然位于细胞
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Preparation of Myristoylated Arf1 and Arf6 Proteins
ADP-ribosylation factor (Arf) proteins were first identified as cofactors for ch
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In Situ Localization of PCR-Amplified DNA and cDNA
In situ hybridization is the only DNA- or RNA-based molecular biology based test
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Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
Abstract The field of lymphatic research has benefited enormously from the recen
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Differential Expression of TIMP-3 During Neoplastic Progression in the Mouse JB6 Model System
The differential display technique has proved to be of particular value in our l