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Counting Chromosomes in Intact Eggs
Chromosomal spreads are an established method to assess ploidy in different cell
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Viral Transformation of Epithelial Cells
Approximately 18% of human cancers have a viral etiology and the majority of the
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The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of P
Sequential activation of kinases within the mitogen-activated protein (MAP) kina
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Genome-Wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq
Chromatin immunoprecipitation (ChIP) is used to evaluate the interaction of prot
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Determination of Proteasomal Activities
The proteasome is an important component of the intracellular system for the tur
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Sequencing-Based Typing of HLA
Human leukocyte antigen (HLA) typing is largely performed by use of PCR-based te
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Tumor Growth Fraction Estimation, Perturbation, and Prognostication
The concept that solid tumors can be comprised of both actively cycling and nonc
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Methods for Studying Spindle Assembly and Chromosome Condensation in Xenopus Egg Extracts
Methods are presented for preparing cytoplasmic extracts from Xenopus laevis egg
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Receptor Knock-Out and Knock-In Strategies
Accumulating examples have demonstrated that knock-out and knock-in mice of G-pr
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Pre-embedding Nanogold Silver and Gold Intensification
Pre-embedding nanogold silver and gold intensification methods involve immunorea
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Purification of Native Bone Marrow Megakaryocytes for Studies of Gene Expression
Megakaryocytes constitute less than 1% of all marrow cells, therefore purificati
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Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization i
Microscopy studies of oxidative stress have often consisted of only immunocytoch
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Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard
Quantitative PCR can be done either by measuring the amount of PCR products at a
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Lysosome Isolation in Isotonic Sucrose
LEVEL IMATERIALS Rat liverPhysiological saline (0.85% w/v NaCl)0.25 M sucrose in
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Assessment of LiposomeCell Interactions
Liposome-cell interactions have been assessed for over 30 years now by an enormo
