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Kinetic Analysis of the Inhibition of Matrix Metalloproteinases: Lessons from the Study of Tissue In
Tissue inhibitors of metalloproteinases (TIMPs) are a group of highly potent inh
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The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
Adhesive interactions between cells regulate tissue integrity as well as the pro
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Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis
Despite rapid growth in our knowledge of potential disease targets following com
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Screens for Mitochondrial Mutants in Yeast
We describe methods that allow isolation, identification, and counting of mitoch
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A number of different viruses interact with the cell cycle in order to subvert host-cell function an
Nonhuman primate embryonic stem (ES) cells are very important for preclinical re
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The Cell Cycle and Virus Infection
A number of different viruses interact with the cell cycle in order to subvert h
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The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference
Embryonic mouse kidneys develop well in organ culture. This, coupled with the fa
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斑块连接 (plaque-bearing junction)
通过细胞质膜内侧的斑块(plaque) 将细胞与细胞、细胞与细胞外基质同细胞骨架连接起来的细胞连接方式称为斑块连接(plaque-bearing junctio
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组织培养细胞染色体制备方法
组织细胞用于遗传学分析最常见的是体外培养的细胞株，多为恶性肿瘤细胞株，且呈贴壁生长，仅小数细胞株为悬浮生长。培养细胞有来源易得、细胞分裂率高和染色体标本制出清晰
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In vitro culture of embryonic lungs
Isolation of Lung Bud EndodermWhat you need:E11-12 mouse embryosDMEM with 5% fet
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In Vitro Generation of Lymphocytes From Embryonic Stem Cells
Lymphocytes arise during ontogeny via a series of increasingly restricted interm
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冰冻断裂复型(freeze-fracture replication)技术
先将生物样品在液氮中(-196℃)进行快速冷冻，防止形成冰晶。然后将冷冻的样品迅速转移到冷冻装置中，并迅速抽成真空。在真空条件下，用冰刀横切冰冻样品，使样品内层
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Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles
The use of enzymes in industrial or medical applications has been limited (excep
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Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
T cells typically differentiate via a series of coordinated steps within the hig
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Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abun
Numerous methods have been published for the detection of protein using avidin–b
