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ENU-Based Phenotype-Driven Screening
Deciphering the contribution of individual genes and in turn pathways to cellula
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Image Analysis of Chromatin Remodelling
Chromatin packaging plays a significant role in regulating gene transcription. S
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Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes
Xenopus oocytes have been utilized in a number of laboratories as an experimenta
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Activation of p53 Protein Function in Response to Cellular Irradiation
p53 protein is a key regulatory component of a stress-inducible cell-cycle check
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Cryopreservation of Mouse Embryos
For almost two decades, introduction of new genes, transgenic technology, has be
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Isolation of Messenger RNA
mRNA comprises approx l–5% of total cellular RNA. Although the actual amount dep
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Using High-Content Microscopy to Study Gonadotrophin-Releasing Hormone Regulation of ERK
Gonadotrophin-releasing hormone (GnRH) is a hypothalamic peptide that acts via G
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Measuring the Inflammasome
Inflammasomes are multiprotein complexes whose activity has been implicated in p
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Determination of Total Homocysteine in Plasma Using Liquid Chromatography Coupled to Tandem Mass Spe
A detailed protocol using liquid chromatography coupled to tandem mass spectrome
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Animal Models of Neurodegenerative Diseases
Animal models of neurodegenerative disease are excellent tools for studying path
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Detection of an mRNA Polymorphism by Differential Display
Differential display technology was utilized to compare programs of gene express
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Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Lipoplex preparations are heterogeneous mixtures of lipoplex particles of differ
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Physical Structure of Nuclear Receptor-DNA Complexes
Nuclear receptors are organized into distinct functional domains, of which the m
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E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
The E2F-family of transcripion factors exerts fascinating and contrasting functi
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酶切位点的详细步骤
一、酶切位点的选择首先，我们需要选择合适的酶切位点。酶切位点应位于基因的特定位置，以便准确切割和重组。通常，我们会根据基因序列的特性来选择酶切位点。二、准备酶和
