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High-Throughput Screens Based on NAD(P)H Depletion
Screening conditions should simulate the final application as closely as possibl
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Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescentl
Cells communicate via endo- and exocytosis with their environment and neighborin
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细胞转染操作步骤
相关专题转染 ,是将外源性基因导入细胞内的一种专门技术。随着基因与蛋白功能研究的深入,转染目前已成为实验室工作中经常涉及的基本方法。转染大致可分为物理介导、化学
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Probing RNA-Protein Interactions by Psoralen Photocrosslinking
RNA molecules can fold into extensive structures containing regions of double-st
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Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction
Investigation of the in vivo regulation of gene expression in human subcutaneous
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Peptidomics: Identification of Pathogenic and Marker Peptides
Recent years have seen great advances in mass spectrometry and proteomics, the s
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Application of PepFect Peptides for the Delivery of Splice-Correcting Oligonucleotides
One oligonucleotide-based approach that appear very promising for the treatment
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Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver
Oval cells and small hepatocytes (SHs) are well known as hepatic stem/progenitor
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内含信号序列(internal signal sequence)
内含信号序列又称内含信号肽(internal signal peptides),它不位于N-末端,但具信号序列的作用,故称为内含信号序列。它可作为蛋白质共翻译转
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In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods
In this chapter we describe the use of cRNA (riboprobes) in the detection of gen
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Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Proc
Part A. Biosynthesis and Extraction of Phosphoinositides and Phosphoinositols Th
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ENU-Based Phenotype-Driven Screening
Deciphering the contribution of individual genes and in turn pathways to cellula
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Image Analysis of Chromatin Remodelling
Chromatin packaging plays a significant role in regulating gene transcription. S
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Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes
Xenopus oocytes have been utilized in a number of laboratories as an experimenta
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Activation of p53 Protein Function in Response to Cellular Irradiation
p53 protein is a key regulatory component of a stress-inducible cell-cycle check