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Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol
An important component of the stress response, as well as many other biological
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Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization
The actin system is involved in different cellular processes such as exo- and en
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Culture of Human Pancreatic Islet Cells
The pancreatic islet is a microorgan composed of four types of hormone-producing
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通过优化由软件控制的加、减速率离心分离单核细胞
相关专题 概述 如今越来越多的临床与细胞实验室检验项目对快速处理样品提出了更高的要求。对于样品分离的常规离心技术,更是需要具有尽可能快的加速和与之相应的减速,
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Human Embryonic Stem (ES) Cell Protocols――Matrigel Aliquoting and Plating
Aliquoting Matrigel:Day one:Put the sterilized tip box (either 200 ml or 1000 ml
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Conditional Gene Mutagenesis in B-Lineage Cells
Since its first application in mice almost 10 yr ago, the Cre/loxP has become th
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Lentivirus-Mediated Modification of Pluripotent Stem Cells
Relatively safe, HIV-1-based lentiviral vectors have served as an efficient mean
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Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix
The photosynthetic membranes of chloroplasts are responsible for capturing light
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Cryopreservation of Algae
Eukaryotic microalgae and prokaryotic cyanobacteria (blue-green algae) have trad
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Gene Targeting in Human Pluripotent Stem Cells
Targeted homologous recombination (HR) is an essential tool in stem cell biology
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Analysis of Embryonic Vascular Morphogenesis
The circulatory system is the first organ system to develop in the embryo. It is
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Assay of Protein Kinases and Protein Phosphorylation
Part A. Protein Kinase-Mediated Phosphorylation Events Protein kinases (ATP:prot
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The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Pr
This chapter explores the combination of a nonradioactive in situ hybridization
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The Isolation of Membrane Proteoglycans
The proteoglycans (PGs) are a large and varied family of complex macromolecules
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Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases
Polyamine oxidation is the main catabolic process of polyamines. This process is