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Profiling Stem Cells Using Quantitative PCR Protein Assays
Reprogramming human somatic cells to induced pluripotent stem cells is an import
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Evolution of the Wnt Pathways
Wnt proteins mediate the transduction of at least three major signaling pathways
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Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent In Situ Hybridization
Hedgehog (Hh) family members are secreted proteins that can act at short and lon
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Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization
In blood vessel development (vasculogenesis) smooth muscle cells (SMCs) are deri
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Methods to Analyze Cellular Necroptosis
Necroptosis is a mechanism of necrotic cell death induced by external stimuli in
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2,2,6,6-Tetramethylpiperidin-1-Oxyl Probes for Evaluating Oxidative Stress on the Cell Membrane and
Oxidative stress is recognized to be involved in many pathological conditions, s
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Efficient Hepatic Differentiation from Human iPS Cells by Gene Transfer
Establishment of protocols for the differentiation of hepatic cells from human e
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Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
The zebrafish system has many features that are ideal for the study of signal tr
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Detection of Apoptosis in Mammalian Development
Mammalian development is dependent on an intricate orchestration of cell prolife
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Differentiate ES cells into cystic embryoid bodies
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture
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Derivation of Human Parthenogenetic Stem Cell Lines
Pluripotent stem cells (PSCs) derived from parthenogenetically activated human o
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RAPD-PCR with Parasitic Hymenoptera
The parasitic Hymenoptera represent a group of insects that is both taxonomicall
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Screening for Radiation Sensitizers of Drosophila Checkpoint Mutants
Anti-cancer therapy is largely comprised of radiation, surgery, and chemotherapy
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Immobilization of Cells in Polyelectrolyte Complexes
Polyelectrolyte complexes are rapidly formed when oppositely charged polyions ar
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Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studie
Gonadal sex differentiation is an important developmental process, in which a bi
