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Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs
A common approach to the study of gene function in Xenopus embryos is to ectopic
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Experimental Control and Characterization of Autophagy in Drosophila
Insects such as the fruit fly Drosophila melanogaster , which fundamentally reor
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COPⅠ被膜小泡(COPⅠcoated vesicle)
由外被蛋白Ⅰ(coat protein Ⅰ, COP Ⅰ )包裹的小泡。主要介导蛋白质从高尔基体运回内质网,包括从反面高尔基体运向顺面高尔基体,以及将蛋白质从反
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Homeoprotein Intercellular Transfer, the Hidden Face of Cell-Penetrating Peptides
Cell-Penetrating Peptides (CPPs) are small peptides internalized by live cells,
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关于琼脂糖凝胶电泳未出现条带的原因
——实验前：样品制备不当①纯度问题：RNA或DNA样品的纯度不够高，可能含有杂质如蛋白质、糖类或其他核酸，影响电泳结果。②浓度问题：样品浓度过低或过
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Propagation of Human Embryonic Stem Cells on Human Feeder Cells
Human embryonic stem (hES) cell lines are usually derived and propagated on inac
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Methods for Imaging Cell Fates in Hematopoiesis
Modern imaging technologies that allow for in vivo monitoring of cells in intact
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Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice
A considerable number of transgenic or knockout mice in which epidermal keratino
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Multi-Dimensional Capillary Electrophoresis and Chromatography for Proteomic Analysis
Comprehensive two-dimensional liquid chromatography-capillary electrophoresis sy
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Telomerase Inhibition by Synthetic Nucleic Acids and Chemosensitization in Human Bladder Cancer Cell
The knockdown of genes that are over-expressed in cancer, and function in tumor
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给出优化琼脂糖凝胶电泳结果分析的方法
【一】凝胶制备的优化①选择合适的琼脂糖浓度琼脂糖浓度直接影响凝胶的孔径大小和分辨能力。一般来说，浓度在0.8%至2.0%之间较为常用。对于不同大小的DNA片段
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The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions
The intermediate filament (IF) proteins have been recently found as dynamic stru
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探讨琼脂糖凝胶电泳在分子生物学研究中的发展趋势
1. DNA的分离与鉴定大片段DNA的分离：琼脂糖凝胶电泳特别适用于分离大片段的DNA。它能够区分相差100bp的DNA片段，且制备容易，分离范围广。普通琼
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Primary Human Osteoblast Cultures
Osteoblast cultures can be used to investigate the mechanisms of bone formation,
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细胞工程技术(cell engineering)
细胞工程技术是细胞生物学与遗传学的交叉领域，主要利用细胞生物学的原理和方法，结合工程学的技术手段，按照人们预先的设计，有计划地改变或创造细胞遗传性的技术。包括体
