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Isolation and Culture of Adult Mouse Hepatocytes
The liver performs a multitude of functions including the regulation of carbohyd
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Expression of Recombinant ADAMTS in Insect Cells
The “a disintegrin and metalloproteinase with thrombospondin motifs” (ADAMTS) en
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Analysis of Microtubule Assembly Kinetics Using Turbidimetry
Turbidity measurements are rapid and reliable methods to follow the effects of d
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Hematopoietic Progenitor Cell Collection
Hematopoietic progenitor cells can be mobilized from the bone marrow microenviro
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Direct Sequencing with Highly Degenerate and lnosine-Containing Primers
Among the many techniques of cloning new genes, one approach involves degenerate
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Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities
Elongation along the proximal distal axis of the limb, from either the pectoral
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培养物的污染及防止
相关专题 按现代的观念，凡是混入培养环境中对细胞生存产生有害的成分和造成细胞不纯的异物都应该视为污染。根据这一概念，组织培养污染物应包括生物（真菌、细菌 、病
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The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its
Tight junctions (TJs) are intercellular structures in epithelial and endothelial
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Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell T
Monitoring of minimal residual disease (MRD) in patients with acute or chronic m
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Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
The upregulation of stress proteins is an important step in the cellular defense
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体外肝细胞损伤模型建立（CCl4和H2O2）
1 大鼠肝细胞的分离和培养 大鼠4％戊巴比妥麻醉，门静脉插管，先以无钙灌流液灌流，继以37℃通入O2的Ⅳ型胶原酶灌流液继续循环灌流15 min。将肝脏移至一平皿
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Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells
The major limitations in gene transduction to embryonic stem (ES) cells are (1)
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In Vitro Assays for Neural Stem Cell Differentiation
Neural stem cells (NSCs) are an undifferentiated population of cells residing in
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Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitt
Studies of the human genome have prompted the development of several cloning sys
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A Transplant Model for Human Epidermal Skin Regeneration
This protocol describes a technically simple in vivo assay of long-term skin reg
